LOCUS       sequence-57378-e         858 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid is used to reconstitute the complete Type II CRISPR 
            system from S. pyogenes and contains the host factor RNaseIII and 
            tracrRNA..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X, 
            Jiang W, Marraffini LA, Zhang F
  TITLE     Multiplex Genome Engineering Using CRISPR/Cas Systems.
  JOURNAL   Science. 2013 Jan 3.
  PUBMED    23287718
REFERENCE   2  (bases 1 to 858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2013 Jan 3."
COMMENT     Sequence Label: pX097-U6-SpTracrRNA-EF1a-hSpRNaseIII-mCherry
FEATURES             Location/Qualifiers
     source          1..858
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(52..99)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             complement(100..804)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(375..394)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     644..662
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 aattttgtaa tccagaggtt gattatcgat aagcttgata tcgaattctt atttcttttt
       61 cttagcttga ccagctttct tagtagcagc aggacgcttc ttgtacagct cgtccatgcc
      121 gccggtggag tggcggccct cggcgcgttc gtactgttcc acgatggtgt agtcctcgtt
      181 gtgggaggtg atgtccaact tgatgttgac gttgtaggcg ccgggcagct gcacgggctt
      241 cttggccttg taggtggtct tgacctcagc gtcgtagtgg ccgccgtcct tcagcttcag
      301 cctctgcttg atctcgccct tcagggcgcc gtcctcgggg tacatccgct cggaggaggc
      361 ctcccagccc atggtcttct tctgcattac ggggccgtcg gaggggaagt tggtgccgcg
      421 cagcttcacc ttgtagatga actcgccgtc ctgcagggag gagtcctggg tcacggtcac
      481 cacgccgccg tcctcgaagt tcatcacgcg ctcccacttg aagccctcgg ggaaggacag
      541 cttcaagtag tcggggatgt cggcggggtg cttcacgtag gccttggagc cgtacatgaa
      601 ctgaggggac aggatgtccc aggcgaaggg cagggggcca cccttggtca ccttcagctt
      661 ggcggtctgg gtgccctcgt aggggcggcc ctcgccctcg ccctcgatct cgaactcgtg
      721 gccgttcacg gagccctcca tgtgcacctt gaagcgcatg aactccttga tgatggccat
      781 gttatcctcc tcgcccttgc tcacggatcc tacctcgctg agctgagcga gggcgttttt
      841 agcagcgtcc tgctcggc
//
