LOCUS       sequence-57379-e         780 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid is used to reconstitute the complete Type II CRISPR 
            system from S. pyogenes and contains the host factor RNaseIII and 
            tracrRNA..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X, 
            Jiang W, Marraffini LA, Zhang F
  TITLE     Multiplex Genome Engineering Using CRISPR/Cas Systems.
  JOURNAL   Science. 2013 Jan 3.
  PUBMED    23287718
REFERENCE   2  (bases 1 to 780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2013 Jan 3."
COMMENT     Sequence Label: pX097-U6-SpTracrRNA-EF1a-hSpRNaseIII-mCherry
FEATURES             Location/Qualifiers
     source          1..780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gccctcgccc tcgccctcga tctcgaactc gtggccgttc acggagccct ccatgtgcac
       61 cttgaagcgc atgaactcct tgatgatggc catgttatcc tcctcgccct tgctcacgga
      121 tcctacctcg ctgagctgag cgagggcgtt tttagcagcg tcctgctcgg ccagtttttt
      181 tgatttaccc aagcccttcg acaacactgc gccattaaca acgatgctaa cttcgaattg
      241 tttggcgtga gctggtcctt tctcacttat tacctgataa tctattgcta catctccctt
      301 ggtctggaga aattcctgca aacatgtttt atagtccttc actctctcga agtttccctt
      361 ttcgacctga gggatcatca cttgtttcag aaagcggcgg actgcgtcga tccctttgtc
      421 cagtagaagt gcgcccagaa acgcttcaaa gagatcgccc agaattgtat cgcgcctcct
      481 gccgccggac ttctcttcgc cttttcccag cttgatataa gcgtcgaatg agcaaaaacg
      541 actaaagccc gccaggctct cttccctgac tatcatggag cgcagctttg acatgtcccc
      601 ttcggttttc ttagggtatt tggcaaacag atattcagag atgatcagct gtaagacagc
      661 atcccccaga aactccaggc gctcgttgtg gctcacattc agtaggcggt gctcattcgc
      721 gtaggaggtg tgagtgaagg cggtttccag cagggtcagg tcattaaact ggatgtcgaa
//
