LOCUS       sequence-57386-e         875 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Longtine MS, McKenzie A 3rd, Demarini DJ, Shah NG, Wach A, Brachat 
            A, Philippsen P, Pringle JR
  TITLE     Additional modules for versatile and economical PCR-based gene 
            deletion and modification in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 1998 Jul;14(10):953-61.
  PUBMED    9717241
REFERENCE   2  (bases 1 to 875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "1998-07"; volume: "14(10)"; pages: "953-61"
COMMENT     Sequence Label: pFA6a-GFP(S65T)-TRP1
FEATURES             Location/Qualifiers
     source          1..875
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      36..223
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(91..115)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        259..406
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
ORIGIN
        1 gaactataca aatagggcgc gccacttcta aataagcgaa tttcttatga tttatgattt
       61 ttattattaa ataagttata aaaaaaataa gtgtatacaa attttaaagt gactcttagg
      121 ttttaaaacg aaaattctta ttcttgagta actctttcct gtaggtcagg ttgctttctc
      181 aggtatagta tgaggtcgct cttattgacc acacctctac cggcagatcc gctagggata
      241 acagggtaat atagatctgt acaatcttga tccggagctt ttcttttttt gccgattaag
      301 aattaattcg gtcgaaaaaa gaaaaggaga gggccaagag ggagggcatt ggtgactatt
      361 gagcacgtga gtatacgtga ttaagcacac aaaggcagct tggagtatgt ctgttattaa
      421 tttcacaggt agttctggtc cattggtgaa agtttgcggc ttgcagagca cagaggccgc
      481 agaatgtgct ctagattccg atgctgactt gctgggtatt atatgtgtgc ccaatagaaa
      541 gagaacaatt gacccggtta ttgcaaggaa aatttcaagt cttgtaaaag catataaaaa
      601 tagttcaggc actccgaaat acttggttgg cgtgtttcgt aatcaaccta aggaggatgt
      661 tttggctctg gtcaatgatt acggcattga tatcgtccaa ctgcatggag atgagtcgtg
      721 gcaagaatac caagagttcc tcggtttgcc agttattaaa agactcgtat ttccaaaaga
      781 ctgcaacata ctactcagtg cagcttcaca gaaacctcat tcgtttattc ccttgtttga
      841 ttcagaagca ggtgggacag gtgaactttt ggatt
//
