LOCUS       sequence-57388-e         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Yang Y, Goldstein BG, Nakagawa H, Katz JP
  TITLE     Kruppel-like factor 5 activates MEK/ERK signaling via EGFR in 
            primary squamous epithelial cells.
  JOURNAL   FASEB J. 2007 Feb;21(2):543-50. Epub 2006 Dec 8.
  PUBMED    17158781
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FASEB J."; 
            date: "2007-02"; volume: "21(2)"; pages: "543-50. Epub 2006 Dec 8"
COMMENT     Sequence Label: pSR-siKlf5
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     42..58
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     43..59
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    125..143
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(189..208)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     promoter        354..853
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(389..412)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     786..805
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
ORIGIN
        1 gccctcactc cttctctagg cgccggaatt agatcgatct ctcgaggtcg acggtatcga
       61 taagcttaaa aaacatgaac gtcttcctcc ctctcttgaa gggaggaaga cgttcatgtg
      121 gggatctcta tcactgatag ggaacttata agattcccaa atccaaagac atttcacgtt
      181 tatggtgatt tcccagaaca catagcgaca tgcaaatatt gcagggcgcc actcccctgt
      241 ccctcacagc catcttcctg ccagggcgca cgcgcgctgg gtgttcccgc ctagtgacac
      301 tgggcccgcg attccttgga gcgggttgat gacgtcagcg ttcgaattct accgggtagg
      361 ggaggcgctt ttcccaaggc agtctggagc atgcgcttta gcagccccgc tgggcacttg
      421 gcgctacaca agtggcctct ggcctcgcac acattccaca tccaccggta ggcgccaacc
      481 ggctccgttc tttggtggcc ccttcgcgcc accttctact cctcccctag tcaggaagtt
      541 cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac
      601 tagtctcgtg cagatggaca gcaccgctga gcaatggaag cgggtaggcc tttggggcag
      661 cggccaatag cagctttgct ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc
      721 cgggggcggg ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt cctccggagg
      781 cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc
      841 tccgggcctt tcgacctgca gcccaag
//
