LOCUS       sequence-57395-e         889 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Koreishi M, Yu S, Oda M, Honjo Y, Satoh A
  TITLE     CK2 phosphorylates Sec31 and regulates ER-To-Golgi trafficking.
  JOURNAL   PLoS One. 2013;8(1):e54382. doi: 10.1371/journal.pone.0054382. Epub 
            2013 Jan 18.
  PUBMED    23349870
REFERENCE   2  (bases 1 to 889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013"; volume: "8(1):e54382. doi"; pages: " 
            10.1371/journal.pone.0054382. Epub 2013 Jan 18"
COMMENT     Sequence Label: FLAG-Sec31a-4SA #33#630
FEATURES             Location/Qualifiers
     source          1..889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        49..67
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      87..96
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             96..161
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 agctctctgg ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca
       61 ctatagggag acccaagctt ggtaccgcca ccatggacta caaagaccat gacggtgatt
      121 ataaagatca tgatatcgat tacaaggatg acgatgacaa agaattcaag ttaaaggaag
      181 tagatcgtac agccatgcag gcatggagcc ctgcccagaa tcaccccatt tacctagcaa
      241 caggaacatc tgctcagcaa ttggatgcaa catttagtac gaatgcttcc cttgagatat
      301 ttgaattaga cctctctgat ccatccttgg atatgaaatc ttgtgccaca ttctcctctt
      361 ctcacaggta ccacaagttg atttgggggc cttataaaat ggattccaaa ggagatgtct
      421 ctggagttct gattgcaggt ggtgaaaatg gaaatattat tctctatgat ccttctaaaa
      481 ttatagctgg agacaaggaa gttgtgattg cccagaatga caagcatact ggcccagtga
      541 gagccttgga tgtgaacatt ttccagacta atctggtagc ttctggtgct aatgaatctg
      601 aaatctacat atgggatcta aataattttg caaccccaat gacaccagga gccaaaacac
      661 agccgccaga agatatcagc tgcattgcat ggaacagaca agttcagcat attttagcat
      721 cagccagtcc cagtggccgg gccactgtat gggatcttag gaaaaatgag ccaatcatca
      781 aagtcagtga ccatagtaac agaatgcatt gttctgggtt ggcatggcat cctgatgttg
      841 ctactcagat ggtccttgcc tccgaggatg accggttacc agtgatcca
//
