LOCUS       sequence-57400-e         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Koreishi M, Yu S, Oda M, Honjo Y, Satoh A
  TITLE     CK2 phosphorylates Sec31 and regulates ER-To-Golgi trafficking.
  JOURNAL   PLoS One. 2013;8(1):e54382. doi: 10.1371/journal.pone.0054382. Epub 
            2013 Jan 18.
  PUBMED    23349870
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013"; volume: "8(1):e54382. doi"; pages: " 
            10.1371/journal.pone.0054382. Epub 2013 Jan 18"
COMMENT     Sequence Label: FLAG-Sec31a S527/799A #27#905
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      88..97
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             97..162
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct tggtaccgcc accatggact acaaagacca tgacggtgat
      121 tataaagatc atgatatcga ttacaaggat gacgatgaca aagaattcaa gttaaaggaa
      181 gtagatcgta cagccatgca ggcatggagc cctgcccaga atcaccccat ttacctagca
      241 acaggaacat ctgctcagca attggatgca acatttagta cgaatgcttc ccttgagata
      301 tttgaattag acctctctga tccatccttg gatatgaaat cttgtgccac attctcctct
      361 tctcacaggt accacaagtt gatttggggg ccttataaaa tggattccaa aggagatgtc
      421 tctggagttc tgattgcagg tggtgaaaat ggaaatatta ttctctatga tccttctaaa
      481 attatagctg gagacaagga agttgtgatt gcccagaatg acaagcatac tggcccagtg
      541 agagccttgg atgtgaacat tttccagact aatctggtag cttctggtgc taatgaatct
      601 gaaatctaca tatgggatct aaataatttt gcaaccccaa tgacaccagg agccaaaaca
      661 cagccgccag aagatatcag ctgcattgca tggaacagac aagttcagca tattttagca
      721 tcagccagtc ccagtggccg ggccactgta tgggatctta ggaaaaatga gccaatcatc
      781 aaagtcagtg accatagtaa cagaatgcat tgttctgggt
//
