LOCUS       sequence-57403-e         853 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Koreishi M, Yu S, Oda M, Honjo Y, Satoh A
  TITLE     CK2 phosphorylates Sec31 and regulates ER-To-Golgi trafficking.
  JOURNAL   PLoS One. 2013;8(1):e54382. doi: 10.1371/journal.pone.0054382. Epub 
            2013 Jan 18.
  PUBMED    23349870
REFERENCE   2  (bases 1 to 853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013"; volume: "8(1):e54382. doi"; pages: " 
            10.1371/journal.pone.0054382. Epub 2013 Jan 18"
COMMENT     Sequence Label: FLAG-Sec31a W985A #790
FEATURES             Location/Qualifiers
     source          1..853
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     55..74
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        55..73
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      93..102
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             102..167
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 agcagagctc tctggctaac tagaggaacc cactgcttac tggcttatcg aaattaatac
       61 gactcactat agggagaccc aagcttggta ccgccaccat ggactacaaa gaccatgacg
      121 gtgattataa agatcatgat atcgattaca aggatgacga tgacaaagaa ttcaagttaa
      181 aggaagtaga tcgtacagcc atgcaggcat ggagccctgc ccagaatcac cccatttacc
      241 tagcaacagg aacatctgct cagcaattgg atgcaacatt tagtacgaat gcttcccttg
      301 agatatttga attagacctc tctgatccat ccttggatat gaaatcttgt gccacattct
      361 cctcttctca caggtaccac aagttgattt gggggcctta taaaatggat tccaaaggag
      421 atgtctctgg agttctgatt gcaggtggtg aaaatggaaa tattattctc tatgatcctt
      481 ctaaaattat agctggagac aaggaagttg tgattgccca gaatgacaag catactggcc
      541 cagtgagagc cttggatgtg aacattttcc agactaatct ggtagcttct ggtgctaatg
      601 aatctgaaat ctacatatgg gatctaaata attttgcaac cccaatgaca ccaggagcca
      661 aaacacagcc gccagaagat atcagctgca ttgcatggaa cagacaagtt cagcatattt
      721 tagcatcagc cagtcccagt ggccgggcca ctgtatggga tcttaggaaa aatgagccaa
      781 tcatcaaagt cagtgaccat agtaacagaa tgcattgttc tgggttggca tggcatcctg
      841 atgttgctac tca
//
