LOCUS       sequence-57490-e         912 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 912)
  AUTHORS   Houser JR, Ford E, Chatterjea SM, Maleri S, Elston TC, Errede B
  TITLE     An improved short-lived fluorescent protein transcriptional reporter
            for Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2012 Dec;29(12):519-30. doi: 10.1002/yea.2932. Epub 2012 Nov 
            21.
  PUBMED    23172645
REFERENCE   2  (bases 1 to 912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/yea.2932"; 
            journalName: "Yeast"; date: "2012-12"; volume: "29"; issue: "12"; 
            pages: "519-30"
COMMENT     Sequence Label: pNC1125
FEATURES             Location/Qualifiers
     source          1..912
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(110..337)
                     /codon_start=1
                     /product="Saccharomyces cerevisiae ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     primer_bind     complement(746..769)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
ORIGIN
        1 ttgggacaac tccagtgaaa agttcttctc ctttactcgt attgggcgcc agggtggttt
       61 ttcttttcac cagtgagacg ggcaacagcc aagctccgga tccgtgcata ccacctctta
      121 gccttagcac aagatgtaag gtggactcct tctgaatgtt gtaatcagac agcgttctac
      181 cgtcttctag ctgcttaccg gcaaagatca atctttgttg atctggaggg ataccttcct
      241 tgtcttgaat tttcgactta acgttgtcga tggtatcgga agattcaact tccaatgtta
      301 tggttttacc ggtcaaagtc ttgacgaaaa tctgcataat ctattagtta aagtaaagtg
      361 ggagggagag ttcgagagtt cttttcgtat tgtagtagat agaagatata cttatcctta
      421 tccgtacagt tcttgctctt tctgaagtag cactttcgga agaagaaaga taagcaggcg
      481 aattgatccg gtaatttagt gtgtgtattt gtgtttgcgt gtctatagaa gtatagtaat
      541 ttatgctaca aaggacctaa tgtataagga aagaatattt agagaaaaga agaaaacaag
      601 agttttatat acatacagag cacatgcatg ccatatgatc atgtgtcgtc gcacacatat
      661 atatatgcct gtatgtgtca gcactaaagt tgcctggcca tccacgctat atacacgcct
      721 ggcagatccg gggttttttc tccttgacgt taaagtatag aggtatatta acaatttttt
      781 gttgatactt ttattacatt tgaataagaa gtaatacaaa ccgaaaatgt tgaaagtatt
      841 agttaaagtg gttatgcagt ttttgcattt atatatctgt taatagatca aaaatcatcg
      901 cttcgctgat at
//
