LOCUS       sequence-57495-e         856 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Toth DJ, Toth JT, Gulyas G, Balla A, Balla T, Hunyady L, Varnai P
  TITLE     Acute depletion of plasma membrane phosphatidylinositol 
            4,5-bisphosphate impairs specific steps in endocytosis of the 
            G-protein-coupled receptor.
  JOURNAL   J Cell Sci. 2012 May 1;125(Pt 9):2185-97. Epub 2012 Feb 22.
  PUBMED    22357943
REFERENCE   2  (bases 1 to 856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2012-05-1"; volume: "125(Pt 9)"; pages: "2185-97. Epub
            2012 Feb 22"
COMMENT     Sequence Label: PM-FRB-mRFP-T2A-FKBP-5-ptase
FEATURES             Location/Qualifiers
     source          1..856
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             53..106
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      113..122
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(119..121,122..442)
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKLE"
ORIGIN
        1 gtacgagcgc gccgagggcc gccactccac cggcgccgtc gactccggga gtgagggcag
       61 aggaagtctt ctaacatgcg gtgacgtgga ggagaatccc ggcccaccgg tcgccaccat
      121 gggagtgcag gtggaaacca tctccccagg agacgggcgc accttcccca agcgcggcca
      181 gacctgcgtg gtgcactaca ccgggatgct tgaagatgga aagaaatttg attcctcccg
      241 ggacagaaac aagcccttta agtttatgct aggcaagcag gaggtgatcc gaggctggga
      301 agaaggggtt gcccagatga gtgtgggtca gagagccaaa ctgactatat ctccagatta
      361 tgcctatggt gccactgggc acccaggcat catcccacca catgccactc tcgtcttcga
      421 tgtggagctt ctaaaactgg aagctcgagg cgcagcagcg ggagcaggag gagcaggtcg
      481 atcggatctt gcagactaca agctccgcgc gcagccgctc ctggtgcggg cccacagcag
      541 cctgggcccc ggccggccgc ggagccccct ggcctgcgac gactgttccc ttcgctcggc
      601 caaatcctcc ttcagcctcc tggcgcccat ccgcagcaag gacgtccgca gcaggagtta
      661 cctggagggc agcctcctgg ccagcggggc cctgttgggg gcggatgagc tggcccgcta
      721 cttcccagac cggaacgtgg cactcttcgt ggccacctgg aacatgcagg gccagaagga
      781 gctcccgccc agcctggacg agttcctgct cccagccgag gccgactatg cccaggacct
      841 gtatgtcatc ggggtc
//
