LOCUS       sequence-57500-e         869 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Toth DJ, Toth JT, Gulyas G, Balla A, Balla T, Hunyady L, Varnai P
  TITLE     Acute depletion of plasma membrane phosphatidylinositol 
            4,5-bisphosphate impairs specific steps in endocytosis of the 
            G-protein-coupled receptor.
  JOURNAL   J Cell Sci. 2012 May 1;125(Pt 9):2185-97. Epub 2012 Feb 22.
  PUBMED    22357943
REFERENCE   2  (bases 1 to 869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2012-05-1"; volume: "125(Pt 9)"; pages: "2185-97. Epub
            2012 Feb 22"
COMMENT     Sequence Label: PM-FRB-Cerulean-T2A-FKBP-5-ptase
FEATURES             Location/Qualifiers
     source          1..869
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             41..94
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      101..110
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(107..109,110..430)
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKLE"
ORIGIN
        1 tctcggcatg gacgagctgt acaaggtcga ctccgggagt gagggcagag gaagtcttct
       61 aacatgcggt gacgtggagg agaatcccgg cccaccggtc gccaccatgg gagtgcaggt
      121 ggaaaccatc tccccaggag acgggcgcac cttccccaag cgcggccaga cctgcgtggt
      181 gcactacacc gggatgcttg aagatggaaa gaaatttgat tcctcccggg acagaaacaa
      241 gccctttaag tttatgctag gcaagcagga ggtgatccga ggctgggaag aaggggttgc
      301 ccagatgagt gtgggtcaga gagccaaact gactatatct ccagattatg cctatggtgc
      361 cactgggcac ccaggcatca tcccaccaca tgccactctc gtcttcgatg tggagcttct
      421 aaaactggaa gctcgaggcg cagcagcggg agcaggagga gcaggtcgat cggatcttgc
      481 agactacaag ctccgcgcgc agccgctcct ggtgcgggcc cacagcagcc tgggccccgg
      541 ccggccgcgg agccccctgg cctgcgacga ctgttccctt cgctcggcca aatcctcctt
      601 cagcctcctg gcgcccatcc gcagcaagga cgtccgcagc aggagttacc tggagggcag
      661 cctcctggcc agcggggccc tgttgggggc ggatgagctg gcccgctact tcccagaccg
      721 gaacgtggca ctcttcgtgg ccacctggaa catgcagggc cagaaggagc tcccgcccag
      781 cctggacgag ttcctgctcc cagccgaggc cgactatgcc caggacctgt atgtcatcgg
      841 ggtccaggag ggctgttctg acaggcggg
//
