LOCUS       sequence-57502-e         892 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Midelfort KS, Hernandez HH, Lippow SM, Tidor B, Drennan CL, Wittrup 
            KD
  TITLE     Substantial energetic improvement with minimal structural 
            perturbation in a high affinity mutant antibody.
  JOURNAL   J Mol Biol. 2004 Oct 22;343(3):685-701.
  PUBMED    15465055
REFERENCE   2  (bases 1 to 892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol 
            Biol."; date: "2004-10-22"; volume: "343(3)"; pages: "685-701"
COMMENT     Sequence Label: pCT302
FEATURES             Location/Qualifiers
     source          1..892
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     91..114
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     CDS             446..457
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             458..484
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 ctgcataacc actttaacta atactttcaa cattttcggt ttgtattact tcttattcaa
       61 atgtaataaa agtatcaaca aaaaattgtt aatatacctc tatactttaa cgtcaaggag
      121 aaaaaacccc ggatcgaatt ccctacttca tacattttca attaagatgc agttacttcg
      181 ctgtttttca atattttctg ttattgcttc agttttagca caggaactga caactatatg
      241 cgagcaaatc ccctcaccaa ctttagaatc gacgccgtac tctttgtcaa cgactactat
      301 tttggccaac gggaaggcaa tgcaaggagt ttttgaatat tacaaatcag taacgtttgt
      361 cagtaattgc ggttctcacc cctcaacaac tagcaaaggc agccccataa acacacagta
      421 tgtttttaag gacaatagct cgacgattga aggtagatac ccatacgacg ttccagacta
      481 cgctctgcag gctagtggtg gtggtggttc tggtggtggt ggttctggtg gtggtggttc
      541 tgctagcgac gtcgttatga ctcaaacacc actatcactt cctgttagtc taggtgatca
      601 agcctccatc tcttgcagat ctagtcagag ccttgtacac agtaatggaa acacctattt
      661 acgttggtac ctgcagaagc caggccagtc tccaaaggtc ctgatctaca aagtttccaa
      721 ccgattttct ggggtcccag acaggttcag tggcagtgga tcagggacag atttcacact
      781 caagatcagc agagtggagg ctgaggatct gggagtttat ttctgctctc aaagtacaca
      841 tgttccgtgg acgttcggtg gaggcaccaa gcttgaaatt aagtcctctg ct
//
