LOCUS       sequence-57503-e         972 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Chao G, Lau WL, Hackel BJ, Sazinsky SL, Lippow SM, Wittrup KD
  TITLE     Isolating and engineering human antibodies using yeast surface 
            display.
  JOURNAL   Nat Protoc. 2006;1(2):755-68.
  PUBMED    17406305
REFERENCE   2  (bases 1 to 972)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Protoc."; date: "2006"; volume: "1(2)"; pages: "755-68"
COMMENT     Sequence Label: pCTcon2
FEATURES             Location/Qualifiers
     source          1..972
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     92..115
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     CDS             447..458
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             459..485
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             738..767
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 actgcataac cactttaact aatactttca acattttcgg tttgtattac ttcttattca
       61 aatgtaataa aagtatcaac aaaaaattgt taatatacct ctatacttta acgtcaagga
      121 gaaaaaaccc cggatcgaat tccctacttc atacattttc aattaagatg cagttacttc
      181 gctgtttttc aatattttct gttattgctt cagttttagc acaggaactg acaactatat
      241 gcgagcaaat cccctcacca actttagaat cgacgccgta ctctttgtca acgactacta
      301 ttttggccaa cgggaaggca atgcaaggag tttttgaata ttacaaatca gtaacgtttg
      361 tcagtaattg cggttctcac ccctcaacaa ctagcaaagg cagccccata aacacacagt
      421 atgtttttaa ggacaatagc tcgacgattg aaggtagata cccatacgac gttccagact
      481 acgctctgca ggctagtggt ggaggaggct ctggtggagg cggtagcgga ggcggagggt
      541 cggctagctg cggtggcggc ggtactagca aaatttctca ttttttgaaa atggaatctt
      601 tgaattttat tagagctcat actccatata ttaatattta caattgtgaa ccagctaatc
      661 catctgaaaa aaattctcca tctactcaat attgttattc tattcaatct tcccaggtcg
      721 actgcggggg cggatccgaa caaaagctta tttctgaaga ggacttgtaa tagctcgaga
      781 tctgataaca acagtgtaga tgtaacaaaa tcgactttgt tcccactgta cttttagctc
      841 gtacaaaata caatatactt ttcatttctc cgtaaacaac atgttttccc atgtaatatc
      901 cttttctatt tttcgttccg ttaccaactt tacacatact ttatatagct attcacttct
      961 atacactaaa aa
//
