LOCUS       sequence-57506-e         861 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Junio AB, Li X, Massey HC, Nolan TJ, Todd Lamitina S, Sundaram MV, 
            Lok JB
  TITLE     Strongyloides stercoralis: cell- and tissue-specific transgene 
            expression and co-transformation with vector constructs 
            incorporating a common multifunctional 3' UTR.
  JOURNAL   Exp Parasitol. 2008 Feb . 118(2):253-65.
  PUBMED    17945217
REFERENCE   2  (bases 1 to 861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Exp 
            Parasitol. 2008 Feb . 118(2):253-65."
COMMENT     Sequence Label: pAJ02
FEATURES             Location/Qualifiers
     source          1..861
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(320..336)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(320..336)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(333..355)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    344..360
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(368..374,375..392,393..398))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    413..434
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(551..568)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(802..821)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catctaattc aacaagaatt gggacaactc cagtgaaaag ttcttctcct ttactcattt
       61 tttctaccgg taccctcaag ggtcctcctg aaaatgttct atgttatgtt agtatcattc
      121 gaaacatacc tttgggtcct ttggccaatc ccggggatcc tctagagtcg acctgcaggc
      181 atgcaagctt atttcatttc caagttgtta gcgtatccat cgttgtgagt gatttcagcc
      241 atttttaagc tctgaaaatt taaatgagaa tagttagtta gtaagatcat gtttaaactt
      301 acagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
      361 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
      421 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
      481 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
      541 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
      601 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
      661 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
      721 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
      781 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
      841 gctctcctgt tccgaccctg c
//
