LOCUS       sequence-57511-e         764 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 764)
  AUTHORS   Billings NA, Emerson MM, Cepko CL
  TITLE     Analysis of thyroid response element activity during retinal 
            development.
  JOURNAL   PLoS One. 2010  . 5(10):e13739.
  PUBMED    21060789
REFERENCE   2  (bases 1 to 764)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One. 
            2010  . 5(10):e13739."
COMMENT     Sequence Label: TRbeta shRNA #1
FEATURES             Location/Qualifiers
     source          1..764
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     30..49
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             82..759
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             82..759
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(212..230)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     480..499
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     674..697
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 tgccttcttc tttttcctac agctcctggg caacgtgctg gttattgtgc tgtctcatca
       61 ttttggcaaa gaattgatcc gatggcctcc tccgaggacg tcatcaagga gttcatgcgc
      121 ttcaaggtgc gcatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc
      181 gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa gggcggcccc
      241 ctgcccttcg cctgggacat cctgtcccct cagttccagt acggctccaa ggcctacgtg
      301 aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg
      361 gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg
      421 caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc
      481 cccgtaatgc agaagaagac catgggctgg gaggcctcca ccgagcggat gtaccccgag
      541 gacggcgccc tgaagggcga gatcaagatg aggctgaagc tgaaggacgg cggccactac
      601 gacgccgagg tcaagaccac ctacatggcc aagaagcccg tgcagctgcc cggcgcctac
      661 aagaccgaca tcaagctgga catcacctcc cacaacgagg actacaccat cgtggaacag
      721 tacgagcgcg ccgagggccg ccactccacc ggcgcctaag aatt
//
