LOCUS       sequence-57514-e         838 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Billings NA, Emerson MM, Cepko CL
  TITLE     Analysis of thyroid response element activity during retinal 
            development.
  JOURNAL   PLoS One. 2010  . 5(10):e13739.
  PUBMED    21060789
REFERENCE   2  (bases 1 to 838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One. 
            2010  . 5(10):e13739."
COMMENT     Sequence Label: GAPDH shRNA #1
FEATURES             Location/Qualifiers
     source          1..838
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     207..224
                     /label=L4440
                     /note="L4440 vector, forward primer"
     ncRNA           complement(447..461)
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
ORIGIN
        1 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
       61 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
      121 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
      181 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
      241 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc ggatccatcg
      301 ataaaaaagc ttaccgtgct gacaaccgca tgcggaacca gcgcaaggca tcttcggatc
      361 ttcacagcac gcgtaagagg gggaagaaag cttctaaccc cgctattcac caccactagg
      421 catgatagaa actgatctgt ttgttacatc tgtggcttca ctaacaaaca gatcagtttc
      481 tatcgcgctc agcagcacct ctccggaagg catcttctcc agctagcgaa gctgccccga
      541 gccgggacgc actttatggt acctagtata tgtgctgccg aagcgagcac ggactaagag
      601 catcgagact gcggtattta aaggctcatc ctgcaagttc ttttagaaca cagtgactgt
      661 tccgtttctt cccaccaaaa ggctccgctt aatagcacat cttgttaact cttaggcccg
      721 cttagccaat ttgtgacgcg attcggcgta atatttccat attagaagcc cctttttgcc
      781 cgtttccgcg cgcgagggct cctgggtagt tctttatgca aatttggcgc cggcgctc
//
