LOCUS       sequence-57517-e         707 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Billings NA, Emerson MM, Cepko CL
  TITLE     Analysis of thyroid response element activity during retinal 
            development.
  JOURNAL   PLoS One. 2010  . 5(10):e13739.
  PUBMED    21060789
REFERENCE   2  (bases 1 to 707)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One. 
            2010  . 5(10):e13739."
COMMENT     Sequence Label: GAPDH shRNA #2
FEATURES             Location/Qualifiers
     source          1..707
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     201..218
                     /label=L4440
                     /note="L4440 vector, forward primer"
     ncRNA           complement(440..454)
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
ORIGIN
        1 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
       61 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
      121 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
      181 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
      241 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagcggatcc atcgataaaa
      301 aagcttaccg tgctgacaac cgcatgcgga accagcgcaa ggcatcttcg gatcttcaca
      361 gcacgcgtaa gaggggaaga aagctgctaa ccccgctatt caccaccact aggcatctgt
      421 ttgtgtacca ccttacatac atctgtggct tcactatgta aggtggtaca caaacaggcg
      481 ctcagcagca cctctccgga aggcatcttc ctccagctag cgaagctgcc ccgagccggg
      541 acgcacttta tggtacctag tatatgtgct gccgaagcga gcacggacta agagcatcga
      601 gactgcggta tttaaaggct catcctgcaa gttcttttag aacacagtga ctgttccgtt
      661 tcttcccacc aaaaggctcc gcttaatagc acatcttgtt aactctt
//
