LOCUS       sequence-57522-e         881 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Liu DS, Phipps WS, Loh KH, Howarth M, Ting AY
  TITLE     Quantum Dot Targeting with Lipoic Acid Ligase and HaloTag for 
            Single-Molecule Imaging on Living Cells.
  JOURNAL   ACS Nano. 2012 Dec 21;6(12):11080-7. doi: 10.1021/nn304793z. Epub 
            2012 Dec 5.
  PUBMED    23181687
REFERENCE   2  (bases 1 to 881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/nn304793z";
            journalName: "ACS Nano"; date: "2012-12-21- 21"; volume: "6"; issue:
            "12"; pages: "11080-7"
COMMENT     Sequence Label: pCAG-AILR-LplA-ER
FEATURES             Location/Qualifiers
     source          1..881
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      54..63
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             124..150
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             160..183
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ctnctcgggc agtgctggtt ttgtgctgtc tcatcatttt ggcaaagaat tccgccacca
       61 tggtagacag acacactcct gctatgggta ctgctgctct gggttccagg ttccactggt
      121 gactatccat atgatgttcc agattatgct ggggcccagg actacaagga tgacgacgat
      181 aagatgagca ccctgagact gctgatcagc gacagctacg acccctggtt caacctggcc
      241 gtggaggagt gcatcttcag acagatgccc gccacccaga gagtgctgtt cctggccaga
      301 aacgccgaca ccgtggtgat cggcagagcc cagaacccct ggaaggagtg caacatcaga
      361 agaatggagg aggacaacgt gagactggcc agaagaagca gcggcggcgg cgccgtgttc
      421 cacgacctgg gcaacacctg cttcaccttc atggccggca agcccgagta cgacaagacc
      481 atcagcacca gcatcgtgct gaacgccctg aacgccctgg gcgtgagcgc cgaggccagc
      541 ggcagaaacg acctggtggt gaagaccgtg gagggcgaca gaaaggtgag cggcagcgcc
      601 tacagagaga ccaaggacag aggcctccac cacggcaccc tgctgctgaa cgccgacctg
      661 agcagactgg ccaactacct gaaccccgac aagaagaagc tggccgccaa gggcatcacc
      721 agcgtgagaa gcagagtgac caacctgacc gagctgctgc ccggcatcac ccacgagcag
      781 gtgtgcgagg ccatcaccga ngccttcttc gcccactacg gcgagagagt ggaggccgag
      841 atcatcagcc ccaacaagac cccccgaacc tgcccaactt c
//
