LOCUS       sequence-57524-e         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  TITLE     pPS
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pPS810
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     20..36
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     20..36
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             complement(40..753)
                     /codon_start=1
                     /product="Aequorea victoria green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(80..100)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     695..723
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     complement(776..799)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
ORIGIN
        1 gctgcagcct gcngtcgact ctagaactag tggatccttt ttgtatagtt catccatgcc
       61 atgtgtaatc ccagcagctg ttacaaactc aagaaggacc atgtggtctc tcttttcgtt
      121 gggatctttc gaaagggcag attgtgtgga caggtaatgg ttgtctggta aaaggacagg
      181 gccatcgcca attggagtat tttgttgata atggtctgct agttgaacgc ttccatcttc
      241 aatgttgtgt ctaattttga agttaacttt gattccattc ttttgtttgt ctgccatgat
      301 gtatacattg tgtgagttat agttgtattc caatttgtgt ccaagaatgt ttccatcttc
      361 tttaaaatca atacctttta actcgattct attaacaagg gtatcacctt caaacttgac
      421 ttcagcacgt gtcttgtagt tcccgtcatc tttgaaaaat atagttcttt cctgtacata
      481 accttcgggc atggcactct tgaaaaagtc atgccgtttc atatgatccg ggtatcttga
      541 aaagcattga acaccataag agaaagtagt gacaagtgtt ggccatggaa caggtagttt
      601 tccagtagtg caaataaatt taagggtaag ttttccgtat gttgcatcac cttcaccctc
      661 tccactgaca gaaaatttgt gcccattaac atcaccatct aattcaacaa gaattgggac
      721 aactccagtg aagagttctt ctcctttact catagatccg gggttttttc tccttgacgt
      781 taaagtatag aggtatatta acaatttttt gttgatactt ttattacatt tgaataagaa
      841 gtaatacaaa ccgaaaatgt tgaaagtatt agttaaagtg
//
