LOCUS       sequence-57557-e        2691 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2691)
  AUTHORS   Carlson LA, Hurley JH
  TITLE     In vitro reconstitution of the ordered assembly of the endosomal 
            sorting complex required for transport at membrane-bound HIV-1 Gag 
            clusters.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Oct 16;109(42):16928-33. doi: 
            10.1073/pnas.1211759109. Epub 2012 Oct 1.
  PUBMED    23027949
REFERENCE   2  (bases 1 to 2691)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1211759109"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2012-10-16- 16"; volume: "109"; issue: "42"; pages: 
            "16928-33"
COMMENT     Sequence Label: pHisAlix
FEATURES             Location/Qualifiers
     source          1..2691
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             52..72
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 atgtcgtact accatcacca tcaccatcac gattacgata tcccaacgac cgaaaacctg
       61 tattttcagg gcgccatggg atccatggcg acattcatct cggtgcagct gaaaaagacc
      121 tcagaggtgg acctggccaa gccgctggtg aagttcatcc agcagactta cccaagcggc
      181 ggggaagagc aggcccagta ctgccgcgcg gcggaggagc tcagcaagct gcgccgcgcc
      241 gcagtcggtc gtccgctgga caagcacgag ggcgcgctcg agacgctcct gagatattat
      301 gatcagattt gttctattga acccaaattc ccattttctg aaaatcagat ctgcttgaca
      361 tttacctgga aggatgcttt cgataaaggt tcactttttg gaggctctgt aaaactggct
      421 cttgcaagct taggatatga aaagagctgt gtgttgttca attgtgcagc cttagctagc
      481 caaattgcag cagaacagaa cctggataat gatgaaggat tgaaaatcgc tgctaaacat
      541 taccagtttg ctagtggtgc ctttttacat attaaagaga cggttttatc tgccttaagt
      601 cgagagccga ccgtggacat atctccagat actgttggga ccctcagtct tattatgctg
      661 gcacaggctc aagaagtatt ttttttaaaa gccacaagag ataaaatgaa agatgccatc
      721 atagctaaat tggctaatca ggctgcagat tattttggtg atgctttcaa acagtgtcaa
      781 tacaaagata ctctccccaa ggaggtgttc cctgtcttgg ctgcaaagca ctgtatcatg
      841 caggccaatg ctgagtacca tcagtctatc ctggcaaaac agcagaagaa atttggagaa
      901 gaaattgcaa ggttacagca tgcagcagaa ctgattaaaa cagtggcatc tcgctatgat
      961 gaatatgtta atgtgaagga tttttctgac aaaatcaatc gtgcccttgc tgcagcaaag
     1021 aaggataatg acttcattta tcatgatcga gttccagacc ttaaagatct agatcctatt
     1081 ggcaaagcca cacttgtgaa atctaccccg gtcaatgtac ccatcagtca gaaatttact
     1141 gatctgtttg agaagatggt tcccgtgtca gtacagcagt ctttggctgc ctataatcag
     1201 aggaaagccg atttggttaa cagatcaatt gctcagatga gagaagccac cactttggca
     1261 aatggggtgc tagcttccct taatcttcca gcagcaattg aagatgtgtc tggagacact
     1321 gtacctcagt ctatattgac taaatccaga tctgtgattg aacagggagg catccagact
     1381 gttgatcagt tgattaaaga actgcctgaa ttactgcaac gaaatagaga aatcctagat
     1441 gagtcattaa ggttgttgga tgaagaagaa gcaaccgata atgatttaag agcaaaattt
     1501 aaggaacgtt ggcaaaggac accatccaat gaactgtata agcctttaag agcagaggga
     1561 accaacttca gaacagtttt agataaagct gtgcaggcag atggacaagt gaaagaatgt
     1621 taccagtctc atcgtgacac catcgtgctt ttgtgtaagc cagagcctga gctgaatgct
     1681 gccatccctt ctgctaatcc ggcaaagacc atgcagggca gtgaggttgt aaatgtctta
     1741 aaatccttat tgtcaaatct tgatgaagta aagaaggaaa gagagggtct ggagaatgac
     1801 ttgaaatctg tgaattttga catgacaagc aagtttttga cagccctggc tcaagatggt
     1861 gtgataaatg aagaagctct ttctgttact gaactagatc gagtctatgg aggtcttaca
     1921 actaaagtcc aagaatctct aaagaaacag gagggacttc ttaaaaatat tcaggtctca
     1981 catcaggaat tttcaaaaat gaaacaatct aataatgaag ctaacttaag agaagaagtt
     2041 ttgaagaatt tagctactgc atatgacaac tttgttgaac ttgtagctaa tttgaaggaa
     2101 ggcacaaagt tttacaatga gttgactgaa atcctggtca ggttccagaa caaatgcagt
     2161 gatatagttt ttgcacggaa gacagaaaga gatgaactct taaaggactt gcaacaaagc
     2221 attgccagag aacctagtgc tccttcaatt cctacacctg cgtatcagtc ctcaccagca
     2281 ggaggacatg caccaactcc tccaactcca gcgccaagaa ccatgccgcc tactaagccc
     2341 cagcccccag ccaggcctcc accacctgtg cttccagcaa atcgagctcc ttctgctact
     2401 gctccatctc cagtgggggc tgggactgct gcgccagctc catcacaaac gcctggctca
     2461 gctcctcctc cacaggcgca gggaccaccc tatcccacct atccaggata tcctgggtat
     2521 tgccaaatgc ccatgcccat gggctataat ccttatgcgt atggccagta taatatgcca
     2581 tatccaccag tgtatcacca gagtcctgga caggctccat acccgggacc ccagcagcct
     2641 tcatacccct tccctcagcc cccacagcag tcttactatc cacagcagta a
//
