LOCUS       sequence-57559-e        8694 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8694)
  AUTHORS   Sherrill KW, Byrd MP, Van Eden ME, Lloyd RE
  TITLE     BCL-2 translation is mediated via internal ribosome entry during 
            cell stress.
  JOURNAL   J Biol Chem. 2004 Jul 9;279(28):29066-74. Epub 2004 May 3.
  PUBMED    15123638
REFERENCE   2  (bases 1 to 8694)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2004-07-9"; volume: "279(28)"; pages: "29066-74. Epub
            2004 May 3"
COMMENT     Sequence Label: phpBCL2-FL(pA)
FEATURES             Location/Qualifiers
     source          1..8694
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        153..532
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        533..736
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     687..707
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     733..757
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          872..1004
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     987..1006
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1049..1067
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2425..4077
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(2491..2509)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     promoter        complement(4139..4157)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4140..4157)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4177..4194)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4183..4407
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4453..4966
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4540..4559)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4750..4771
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4989..5009)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4994..5324
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5175..5310
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5391..6185
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5445..5464)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6055..6074
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6359..6493
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6396..6415)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6450..6469
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6525..6541)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6525..6541)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6538..6560)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6549..6565
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6573..6579,6580..6597,6598..6603))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6618..6639
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6756..6773)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6927..7515)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7007..7026)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7686..8477,8478..8546))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8309..8328
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8547..8651)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 attaaacctc gatcttcaat attggccatt agccatatta ttcattggtt atatagcata
       61 aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata tgtacattta
      121 tattggctca tgtccaatat gaccgccatg ttggcattga ttattgacta gttattaata
      181 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact
      241 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
      301 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
      361 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtccgccccc
      421 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttacg
      481 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg
      541 gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct
      601 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
      661 atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac ggtgggaggt
      721 ctatataagc agagctcgtt tagtgaaccg tcagatcact agaagcttta ttgcggtagt
      781 ttatcacagt taaattgcta acgcagtcag tgcttctgac acaacagtct cgaacttaag
      841 ctgcagaagt tggtcgtgag gcactgggca ggtaagtatc aaggttacaa gacaggttta
      901 aggagaccaa tagaaactgg gcttgtcgag acagagaaga ctcttgcgtt tctgataggc
      961 acctattggt cttactgaca tccactttgc ctttctctcc acaggtgtcc actcccagtt
     1021 caattacagc tcttaaggct agagtactta atacgactca ctataggcta gcattctgtc
     1081 tgttttgggg gattgcaagt aaagatagga tccggggcgc gtggtggcgg ctgcagccgc
     1141 caccacgcgc cccaagctta tctcaaaagc aagtgtaagc agcagcgccg cagccactgc
     1201 ttttataagc tagccacccg ttcgttgagc gagttctcaa aagtgaacaa taattctaga
     1261 gcggccgcaa aaaataaaac cctcccccac cacctccttc tccccacccc tcgccgcacc
     1321 acacacagcg cgggcttcta gcgctcggca ccggcgggcc aggcgcgtcc tgccttcatt
     1381 tatccagcag cttttcggaa aatgcatttg ctgttcggag tttaatcaga agacgattcc
     1441 tgcctccgtc cccggctcct tcatcgtccc atctcccctg tctctctcct ggggaggcgt
     1501 gaagcggtcc cgtggataga gattcatgcc tgtgtccgcg cgtgtgtgcg cgcgtataaa
     1561 ttgccgagaa ggggaaaaca tcacaggact tctgcgaata ccggactgaa aattgtaatt
     1621 catctgccgc cgccgctgcc tttttttttt ctcgagctct tgagatctcc ggttgggatt
     1681 cctgcggatt gacatttctg tgaagcagaa gtctgggaat cgatctggaa atcctcctaa
     1741 tttttactcc ctctcccccc gactcctgat tcattgggaa gtttcaaatc agctataact
     1801 ggagagtgct gaagattgat gggatcgttg ccttatgcat ttgttttggt tttacaaaaa
     1861 ggaaacttga cagaggatca tgctgtactt aaaaaataca agtaagtctc gcacaggaaa
     1921 ttggtttaat gtaactttca atggaaacct ttgagatttt ttacttaaag tgcattcgag
     1981 taaatttaat ttccaggcag cttaatacat tgtttttagc cgtgttactt gtagtgtgta
     2041 tgccctgctt tcactcagtg tgtacaggga aacgcacctg attttttact tattagtttg
     2101 ttttttcttt aacctttcag catcacagag gaagtagact gatattaaca atacttacta
     2161 ataataacgt gcctcatgaa ataaagatcc gaaaggaatt ggaataaaaa tttcctgcgt
     2221 ctcatgccaa gagggaaaca ccagaatcaa gtgttccgcg tgattgaaga caccccctcg
     2281 tccaagaatg caaagcacat ccaataaaat agctggatta taactcctct tctttctctg
     2341 ggggccgtgg ggtgggagct ggggcgagag gtgccgttgg cccccgttgc ttttcttctg
     2401 ggaagcatgg gcccacagga cgtcatggaa gacgccaaaa acataaagaa aggcccggcg
     2461 ccattctatc ctctagagga tggaaccgct ggagagcaac tgcataaggc tatgaagaga
     2521 tacgccctgg ttcctggaac aattgctttt acagatgcac atatcgaggt gaacatcacg
     2581 tacgcggaat acttcgaaat gtccgttcgg ttggcagaag ctatgaaacg atatgggctg
     2641 aatacaaatc acagaatcgt cgtatgcagt gaaaactctc ttcaattctt tatgccggtg
     2701 ttgggcgcgt tatttatcgg agttgcagtt gcgcccgcga acgacattta taatgaacgt
     2761 gaattgctca acagtatgaa catttcgcag cctaccgtag tgtttgtttc caaaaagggg
     2821 ttgcaaaaaa ttttgaacgt gcaaaaaaaa ttaccaataa tccagaaaat tattatcatg
     2881 gattctaaaa cggattacca gggatttcag tcgatgtaca cgttcgtcac atctcatcta
     2941 cctcccggtt ttaatgaata cgattttgta ccagagtcct ttgatcgtga caaaacaatt
     3001 gcactgataa tgaattcctc tggatctact gggttaccta agggtgtggc ccttccgcat
     3061 agaactgcct gcgtcagatt ctcgcatgcc agagatccta tttttggcaa tcaaatcatt
     3121 ccggatactg cgattttaag tgttgttcca ttccatcacg gttttggaat gtttactaca
     3181 ctcggatatt tgatatgtgg atttcgagtc gtcttaatgt atagatttga agaagagctg
     3241 tttttacgat cccttcagga ttacaaaatt caaagtgcgt tgctagtacc aaccctattt
     3301 tcattcttcg ccaaaagcac tctgattgac aaatacgatt tatctaattt acacgaaatt
     3361 gcttctgggg gcgcacctct ttcgaaagaa gtcggggaag cggttgcaaa acgcttccat
     3421 cttccaggga tacgacaagg atatgggctc actgagacta catcagctat tctgattaca
     3481 cccgaggggg atgataaacc gggcgcggtc ggtaaagttg ttccattttt tgaagcgaag
     3541 gttgtggatc tggataccgg gaaaacgctg ggcgttaatc agagaggcga attatgtgtc
     3601 agaggaccta tgattatgtc cggttatgta aacaatccgg aagcgaccaa cgccttgatt
     3661 gacaaggatg gatggctaca ttctggagac atagcttact gggacgaaga cgaacacttc
     3721 ttcatagttg accgcttgaa gtctttaatt aaatacaaag gatatcaggt ggcccccgct
     3781 gaattggaat cgatattgtt acaacacccc aacatcttcg acgcgggcgt ggcaggtctt
     3841 cccgacgatg acgccggtga acttcccgcc gccgttgttg ttttggagca cggaaagacg
     3901 atgacggaaa aagagatcgt ggattacgtg gccagtcaag taacaaccgc gaaaaagttg
     3961 cgcggaggag ttgtgtttgt ggacgaagta ccgaaaggtc ttaccggaaa actcgacgca
     4021 agaaaaatca gagagatcct cataaaggcc aagaagggcg gaaagtccaa attgtaagga
     4081 tccgggccca aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaccggtg ggccctattc
     4141 tatagtgtca cctaaatgct agagctcgct gatcagcctc gactgtgcct tctagttgcc
     4201 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
     4261 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
     4321 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
     4381 atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcga
     4441 ggggggatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     4501 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     4561 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggc atccctttag
     4621 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     4681 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     4741 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     4801 cttttgattt ataagggatt ttggggattt cggcctattg gttaaaaaat gagctgattt
     4861 aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttaa atatttgctt
     4921 atacaatctt cctgtttttg gggcttttct gattatcaac cggggtgggt accgagctcg
     4981 aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc caggcaggca
     5041 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
     5101 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
     5161 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     5221 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     5281 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt
     5341 ggatatccat tttcggatct gatcaagaga caggatgagg atcgtttcgc atgattgaac
     5401 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
     5461 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
     5521 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg
     5581 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
     5641 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
     5701 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
     5761 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
     5821 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
     5881 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc
     5941 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
     6001 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
     6061 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
     6121 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
     6181 tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg
     6241 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
     6301 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa
     6361 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     6421 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     6481 tcatgtctgg atcccgtcga cctcgagagc ttggcgtaat catggtcata gctgtttcct
     6541 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     6601 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     6661 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     6721 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     6781 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     6841 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     6901 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     6961 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     7021 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     7081 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat
     7141 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     7201 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     7261 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     7321 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     7381 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     7441 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     7501 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     7561 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     7621 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     7681 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     7741 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     7801 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     7861 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     7921 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     7981 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     8041 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     8101 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     8161 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     8221 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     8281 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     8341 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     8401 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     8461 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     8521 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     8581 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     8641 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc ggga
//
