LOCUS       sequence-57562-e        9595 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9595)
  AUTHORS   Sherrill KW, Byrd MP, Van Eden ME, Lloyd RE
  TITLE     BCL-2 translation is mediated via internal ribosome entry during 
            cell stress.
  JOURNAL   J Biol Chem. 2004 Jul 9;279(28):29066-74. Epub 2004 May 3.
  PUBMED    15123638
REFERENCE   2  (bases 1 to 9595)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2004-07-9"; volume: "279(28)"; pages: "29066-74. Epub
            2004 May 3"
COMMENT     Sequence Label: phpRL-BCL2-FL-pA
FEATURES             Location/Qualifiers
     source          1..9595
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        153..532
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        533..736
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     687..707
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     733..757
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          872..1004
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     987..1006
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1049..1067
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1219..2154
                     /codon_start=1
                     /product="luciferase from the anthozoan coelenterate 
                     Renilla reniformis (sea pansy)"
                     /label=Rluc
                     /translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     CDS             3326..4978
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(3392..3410)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     promoter        complement(5040..5058)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5041..5058)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5078..5095)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5084..5308
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5354..5867
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5441..5460)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5651..5672
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5890..5910)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5895..6225
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6076..6211
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6292..7086
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6346..6365)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6956..6975
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7260..7394
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7297..7316)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7351..7370
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7426..7442)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7426..7442)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7439..7461)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7450..7466
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7474..7480,7481..7498,7499..7504))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7519..7540
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7657..7674)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7828..8416)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7908..7927)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8587..9378,9379..9447))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9210..9229
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9448..9552)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 attaaacctc gatcttcaat attggccatt agccatatta ttcattggtt atatagcata
       61 aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata tgtacattta
      121 tattggctca tgtccaatat gaccgccatg ttggcattga ttattgacta gttattaata
      181 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact
      241 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
      301 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
      361 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtccgccccc
      421 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttacg
      481 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg
      541 gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct
      601 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
      661 atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac ggtgggaggt
      721 ctatataagc agagctcgtt tagtgaaccg tcagatcact agaagcttta ttgcggtagt
      781 ttatcacagt taaattgcta acgcagtcag tgcttctgac acaacagtct cgaacttaag
      841 ctgcagaagt tggtcgtgag gcactgggca ggtaagtatc aaggttacaa gacaggttta
      901 aggagaccaa tagaaactgg gcttgtcgag acagagaaga ctcttgcgtt tctgataggc
      961 acctattggt cttactgaca tccactttgc ctttctctcc acaggtgtcc actcccagtt
     1021 caattacagc tcttaaggct agagtactta atacgactca ctataggcta gcattctgtc
     1081 tgttttgggg gattgcaagt aaagatagga tccggggcgc gtggtggcgg ctgcagccgc
     1141 caccacgcgc cccaagctta tctcaaaagc aagtgtaagc agcagcgccg cagccactgc
     1201 ttttataagc tagccaccat gacttcgaaa gtttatgatc cagaacaaag gaaacggatg
     1261 ataactggtc cgcagtggtg ggccagatgt aaacaaatga atgttcttga ttcatttatt
     1321 aattattatg attcagaaaa acatgcagaa aatgctgtta tttttttaca tggtaacgcg
     1381 gcctcttctt atttatggcg acatgttgtg ccacatattg agccagtagc gcggtgtatt
     1441 ataccagacc ttattggtat gggcaaatca ggcaaatctg gtaatggttc ttataggtta
     1501 cttgatcatt acaaatatct tactgcatgg tttgaacttc ttaatttacc aaagaagatc
     1561 atttttgtcg gccatgattg gggtgcttgt ttggcatttc attatagcta tgagcatcaa
     1621 gataagatca aagcaatagt tcacgctgaa agtgtagtag atgtgattga atcatgggat
     1681 gaatggcctg atattgaaga agatattgcg ttgatcaaat ctgaagaagg agaaaaaatg
     1741 gttttggaga ataacttctt cgtggaaacc atgttgccat caaaaatcat gagaaagtta
     1801 gaaccagaag aatttgcagc atatcttgaa ccattcaaag agaaaggtga agttcgtcgt
     1861 ccaacattat catggcctcg tgaaatcccg ttagtaaaag gtggtaaacc tgacgttgta
     1921 caaattgtta ggaattataa tgcttatcta cgtgcaagtg atgatttacc aaaaatgttt
     1981 attgaatcgg acccaggatt cttttccaat gctattgttg aaggtgccaa gaagtttcct
     2041 aatactgaat ttgtcaaagt aaaaggtctt catttttcgc aagaagatgc acctgatgaa
     2101 atgggaaaat atatcaaatc gttcgttgag cgagttctca aaaatgaaca ataattctag
     2161 agcggccgca aaaaataaaa ccctccccca ccacctcctt ctccccaccc ctcgccgcac
     2221 cacacacagc gcgggcttct agcgctcggc accggcgggc caggcgcgtc ctgccttcat
     2281 ttatccagca gcttttcgga aaatgcattt gctgttcgga gtttaatcag aagacgattc
     2341 ctgcctccgt ccccggctcc ttcatcgtcc catctcccct gtctctctcc tggggaggcg
     2401 tgaagcggtc ccgtggatag agattcatgc ctgtgtccgc gcgtgtgtgc gcgcgtataa
     2461 attgccgaga aggggaaaac atcacaggac ttctgcgaat accggactga aaattgtaat
     2521 tcatctgccg ccgccgctgc cttttttttt tctcgagctc ttgagatctc cggttgggat
     2581 tcctgcggat tgacatttct gtgaagcaga agtctgggaa tcgatctgga aatcctccta
     2641 atttttactc cctctccccc cgactcctga ttcattggga agtttcaaat cagctataac
     2701 tggagagtgc tgaagattga tgggatcgtt gccttatgca tttgttttgg ttttacaaaa
     2761 aggaaacttg acagaggatc atgctgtact taaaaaatac aagtaagtct cgcacaggaa
     2821 attggtttaa tgtaactttc aatggaaacc tttgagattt tttacttaaa gtgcattcga
     2881 gtaaatttaa tttccaggca gcttaataca ttgtttttag ccgtgttact tgtagtgtgt
     2941 atgccctgct ttcactcagt gtgtacaggg aaacgcacct gattttttac ttattagttt
     3001 gttttttctt taacctttca gcatcacaga ggaagtagac tgatattaac aatacttact
     3061 aataataacg tgcctcatga aataaagatc cgaaaggaat tggaataaaa atttcctgcg
     3121 tctcatgcca agagggaaac accagaatca agtgttccgc gtgattgaag acaccccctc
     3181 gtccaagaat gcaaagcaca tccaataaaa tagctggatt ataactcctc ttctttctct
     3241 gggggccgtg gggtgggagc tggggcgaga ggtgccgttg gcccccgttg cttttcttct
     3301 gggaagcatg ggcccacagg acgtcatgga agacgccaaa aacataaaga aaggcccggc
     3361 gccattctat cctctagagg atggaaccgc tggagagcaa ctgcataagg ctatgaagag
     3421 atacgccctg gttcctggaa caattgcttt tacagatgca catatcgagg tgaacatcac
     3481 gtacgcggaa tacttcgaaa tgtccgttcg gttggcagaa gctatgaaac gatatgggct
     3541 gaatacaaat cacagaatcg tcgtatgcag tgaaaactct cttcaattct ttatgccggt
     3601 gttgggcgcg ttatttatcg gagttgcagt tgcgcccgcg aacgacattt ataatgaacg
     3661 tgaattgctc aacagtatga acatttcgca gcctaccgta gtgtttgttt ccaaaaaggg
     3721 gttgcaaaaa attttgaacg tgcaaaaaaa attaccaata atccagaaaa ttattatcat
     3781 ggattctaaa acggattacc agggatttca gtcgatgtac acgttcgtca catctcatct
     3841 acctcccggt tttaatgaat acgattttgt accagagtcc tttgatcgtg acaaaacaat
     3901 tgcactgata atgaattcct ctggatctac tgggttacct aagggtgtgg cccttccgca
     3961 tagaactgcc tgcgtcagat tctcgcatgc cagagatcct atttttggca atcaaatcat
     4021 tccggatact gcgattttaa gtgttgttcc attccatcac ggttttggaa tgtttactac
     4081 actcggatat ttgatatgtg gatttcgagt cgtcttaatg tatagatttg aagaagagct
     4141 gtttttacga tcccttcagg attacaaaat tcaaagtgcg ttgctagtac caaccctatt
     4201 ttcattcttc gccaaaagca ctctgattga caaatacgat ttatctaatt tacacgaaat
     4261 tgcttctggg ggcgcacctc tttcgaaaga agtcggggaa gcggttgcaa aacgcttcca
     4321 tcttccaggg atacgacaag gatatgggct cactgagact acatcagcta ttctgattac
     4381 acccgagggg gatgataaac cgggcgcggt cggtaaagtt gttccatttt ttgaagcgaa
     4441 ggttgtggat ctggataccg ggaaaacgct gggcgttaat cagagaggcg aattatgtgt
     4501 cagaggacct atgattatgt ccggttatgt aaacaatccg gaagcgacca acgccttgat
     4561 tgacaaggat ggatggctac attctggaga catagcttac tgggacgaag acgaacactt
     4621 cttcatagtt gaccgcttga agtctttaat taaatacaaa ggatatcagg tggcccccgc
     4681 tgaattggaa tcgatattgt tacaacaccc caacatcttc gacgcgggcg tggcaggtct
     4741 tcccgacgat gacgccggtg aacttcccgc cgccgttgtt gttttggagc acggaaagac
     4801 gatgacggaa aaagagatcg tggattacgt ggccagtcaa gtaacaaccg cgaaaaagtt
     4861 gcgcggagga gttgtgtttg tggacgaagt accgaaaggt cttaccggaa aactcgacgc
     4921 aagaaaaatc agagagatcc tcataaaggc caagaagggc ggaaagtcca aattgtaagg
     4981 atccgggccc aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaccggt gggccctatt
     5041 ctatagtgtc acctaaatgc tagagctcgc tgatcagcct cgactgtgcc ttctagttgc
     5101 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     5161 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
     5221 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
     5281 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctcg
     5341 aggggggatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     5401 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     5461 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg catcccttta
     5521 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     5581 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     5641 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     5701 tcttttgatt tataagggat tttggggatt tcggcctatt ggttaaaaaa tgagctgatt
     5761 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaattta aatatttgct
     5821 tatacaatct tcctgttttt ggggcttttc tgattatcaa ccggggtggg taccgagctc
     5881 gaattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccaggcaggc
     5941 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     6001 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
     6061 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     6121 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     6181 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct
     6241 tggatatcca ttttcggatc tgatcaagag acaggatgag gatcgtttcg catgattgaa
     6301 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac
     6361 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg
     6421 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag
     6481 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt
     6541 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg
     6601 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg
     6661 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga
     6721 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag
     6781 gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat
     6841 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt
     6901 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg
     6961 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt
     7021 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
     7081 ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac
     7141 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
     7201 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca
     7261 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     7321 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     7381 atcatgtctg gatcccgtcg acctcgagag cttggcgtaa tcatggtcat agctgtttcc
     7441 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     7501 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     7561 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     7621 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     7681 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     7741 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     7801 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     7861 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     7921 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     7981 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct caatgctcac gctgtaggta
     8041 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     8101 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     8161 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     8221 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
     8281 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     8341 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     8401 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     8461 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     8521 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     8581 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     8641 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     8701 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     8761 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     8821 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     8881 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     8941 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     9001 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     9061 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     9121 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     9181 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     9241 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     9301 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     9361 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     9421 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     9481 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     9541 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat cggga
//
