LOCUS       sequence-57580-e        3784 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3784)
  AUTHORS   Korta DZ, Tuck S, Hubbard EJ
  TITLE     S6K links cell fate, cell cycle and nutrient response in C. elegans 
            germline stem/progenitor cells.
  JOURNAL   Development. 2012 Mar;139(5):859-70. doi: 10.1242/dev.074047. Epub 
            2012 Jan 25.
  PUBMED    22278922
REFERENCE   2  (bases 1 to 3784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/dev.074047"; journalName: "Development"; date: "2012-03"; 
            volume: "139"; issue: "5"; pages: "859-70"
COMMENT     Sequence Label: pGC480
FEATURES             Location/Qualifiers
     source          1..3784
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        19..37
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1175..1191)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1176..1192)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(1217..1235)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1245..1262)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1245..1261)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1254..1276)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1403..1858
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1490..1509)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1700..1721
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1884..1988
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1989..2057,2058..2849)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2207..2226)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3020..3608
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3509..3528
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3762..3779
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 aacctggctt atcgaaatta atacgactca ctatagggag accggcagat ctgatatcat
       61 cgatgaattc gagctccacc gcggtggcgg ccgctctaga caagccattc aacaccttct
      121 tcttcgtctt ctttcaaaaa cttttgttta ataaactgtg aggattcaca tctgatgagt
      181 tcgaatcgtt gatcagaaat tcgaagaagt tcattgagaa tcagggcgac ggcatgccat
      241 ctatccaaat cgtccttttg cgatgggttt ggttgacctt ccagtgcttc atcgaaacat
      301 ttcttaaacc actcagtttt attctttgct tcacgctgtg aaacaattgt caaaaccacg
      361 tgtaatgcat caattccggc gattcttaca gctggattct tgtctctgac agcgttgaag
      421 atatacttga aaaataagtt tacacgaaga aagaatgctg tcggtgtggc cagtgcgagc
      481 tcacgagaga ggtgtgaagc ggcgagtcga cgaatttgat caatttcctg ttgatttttg
      541 ggttgctgac tatgtggaac attttgacca agccattcgt ggcaatgatc aagacaacga
      601 tcaaccagct cggccgcgta tgatttacaa gtcgcaatca gaaaagcgag agcttttgaa
      661 gccatcttga ctgtatcttc atctaatcca tttccattat ttaacatttt cagaagataa
      721 ttcgcatatc gaataacatt tccagaatga gtttcagcta agcacacgat gagatagata
      781 ccggctcgtt tctgatcgat atttgaattc gacttcatac aattataaat tgcactctga
      841 gaggctatat ctgttcgtcc atcgattgca ttcaggaaag catcactaaa agtatttggc
      901 tcgtctttca gctcacttcg aacatatctg ctgagttctc gagcagcctg tcgttgattt
      961 gcatctctat tttccgtaat tcggctccga tattgtgaag caagagctct tgcagcatca
     1021 atcccttgtt tacgatgccg ctcgtcaaat tcatttgtag ctggagcgga gactattcga
     1081 ttggaagttg tggctgcttt gttctgtcta tcggcattca tttgaaaact aattccgtgt
     1141 tgttggagct gcaggaattc gatatcaagc ttatcgatac cgtcgacctc gagggggggc
     1201 ccggtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta
     1261 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
     1321 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     1381 cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     1441 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     1501 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     1561 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     1621 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     1681 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     1741 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     1801 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag
     1861 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     1921 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     1981 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     2041 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     2101 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     2161 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     2221 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     2281 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     2341 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     2401 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     2461 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     2521 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     2581 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     2641 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     2701 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     2761 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     2821 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     2881 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     2941 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     3001 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     3061 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     3121 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     3181 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     3241 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     3301 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     3361 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     3421 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     3481 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     3541 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     3601 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     3661 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     3721 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     3781 aagc
//
