LOCUS       sequence-57581-e        3947 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3947)
  AUTHORS   Korta DZ, Tuck S, Hubbard EJ
  TITLE     S6K links cell fate, cell cycle and nutrient response in C. elegans 
            germline stem/progenitor cells.
  JOURNAL   Development. 2012 Mar;139(5):859-70. doi: 10.1242/dev.074047. Epub 
            2012 Jan 25.
  PUBMED    22278922
REFERENCE   2  (bases 1 to 3947)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/dev.074047"; journalName: "Development"; date: "2012-03"; 
            volume: "139"; issue: "5"; pages: "859-70"
COMMENT     Sequence Label: pGC479
FEATURES             Location/Qualifiers
     source          1..3947
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        19..37
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1338..1354)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1339..1355)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(1380..1398)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1408..1425)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1408..1424)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1417..1439)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1566..2021
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1653..1672)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1863..1884
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2047..2151
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2152..2220,2221..3012)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2370..2389)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3183..3771
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3672..3691
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3925..3942
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 aacctggctt atcgaaatta atacgactca ctatagggag accggcagat ctgatatcat
       61 cgatgaattc gagctccacc gcggtggcgg ccgactttgg ctagactacg gtgttgacga
      121 aggaacacct ccactccaac ttccgatggt tcttcaagta cttctgagtc aggcacatcg
      181 tgtcagagct ctacgattac ttgcacgatt ccttgatttt ggtagatggg ctatcggata
      241 ttcacttgcc gttggaatct ttccatatgt tctgaagcta cttcaaagca atattagtga
      301 tttgaaaatc tctttagcgt ttatttgggc aaaaattctg gcagtagatc catcgtgtca
      361 aatggaatta ttcaatgagt gtatcgatga atcagcaaca caacaacaat caccagcaca
      421 atcacaacaa ccatcagttg cattgaaacc atcatccatg tttgcaaatc atccaccacc
      481 tgcacaatta acaatggata aacgtgcact tcagattaat actcaaactg gcggaagatt
      541 tcaaactaat cagaatcagg cattggatat gcaattacaa tatgctcaac aacaagctca
      601 tcagaggcaa atggatccat caggaggaca acattcacca aatgctcagc ctagacaacc
      661 acaaccacag caaccacaac aactacaaca gcaaccatca cagcaacaac aacaacaatc
      721 tagacaaccg caaggaatta tgaaaccaac gaatgcacag ccacaagaaa agaagacaat
      781 gcttcgtttt caatatttcc ttcaacttct cggcgatcct gaaatcaaac cgaaacagaa
      841 aactgttgct gcattcgtac ttgctcagct aacatccaat aatttcaaac tggcacagaa
      901 agagctcacc aataagggat acatgggaat ttgtacggaa ttgatggtcg atgatactgc
      961 gagaagcgtc aaattgctga aactgtggat tctgattgga ttgggtcgtc tgtggtcaga
     1021 ttatgatgta gccagatggc agggtattcg attaatggct catgataaaa tgattttgga
     1081 attgagtgat gaatcagcag aagttcgtgc agctgctgta tttgcacttg gaagcttatt
     1141 gaggaactct tcgagatcaa atgagcatgc atcagcagtt gaagaaaatc tagcaggaga
     1201 actatgttcc cagtgtgtgt ttgatagttc tgttcttgtg agagaagaac tgattgttgc
     1261 tcttcaatgg tttgtgtttg atttcgagaa acgattcgtg aaattcttac tctgcaggaa
     1321 ttcgatatca agcttatcga taccgtcgac ctcgaggggg ggcccggtac ccaattcgcc
     1381 ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga
     1441 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
     1501 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
     1561 atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     1621 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     1681 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     1741 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     1801 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     1861 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     1921 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     1981 atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga
     2041 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     2101 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     2161 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     2221 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     2281 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     2341 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     2401 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     2461 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     2521 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     2581 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     2641 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     2701 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     2761 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     2821 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     2881 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     2941 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     3001 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     3061 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     3121 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     3181 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     3241 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     3301 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
     3361 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     3421 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     3481 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     3541 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     3601 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     3661 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     3721 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     3781 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     3841 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     3901 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagc
//
