LOCUS       sequence-57601-e         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Ding Y, Ai HW, Hoi H, Campbell RE
  TITLE     Forster resonance energy transfer-based biosensors for 
            multiparameter ratiometric imaging of Ca2+ dynamics and caspase-3 
            activity in single cells.
  JOURNAL   Anal Chem. 2011 Dec 15;83(24):9687-93. doi: 10.1021/ac202595g. Epub 
            2011 Nov 28.
  PUBMED    22080726
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/ac202595g";
            journalName: "Anal Chem"; date: "2011-12-15- 15"; volume: "83"; 
            issue: "24"; pages: "9687-93"
COMMENT     Sequence Label: pcpmAmetrine
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             82..99
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             103..135
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(139..147,148..162)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     primer_bind     complement(439..460)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 tgagcttttt tcgcaactct ctactgtttc tccatacccg tttttttggg ctaacaggag
       61 gaattaacca tggggggttc tcatcatcat catcatcatg gtatggctag catgactggt
      121 ggacagcaaa tgggtcggga tctgtacgac gatgacgata aggatccgag ctcgagcaag
      181 gacggcggcg tgcagctcgc cgacgtgtac cagcagaaca cgcccatcgg cgacggctcc
      241 gtgctgctgc ctgacaacca ctacctgagc ttccggagca agctgttcaa agaccccaac
      301 gagcagcgcg atcacatggt cctgctggag ttcgttaccg ccgccgggat cactcccggc
      361 atggacgagc tgtacaaggg cggatccggc ggaatggtga gcaagggcga ggagctgttc
      421 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
      481 gtgcgtggcg ggggcgaggg cgatgccacc aacggcaagc tgaccctgaa gttcatctgc
      541 acctccggca agctgcccgt gccctggccc accctcgtga ccaccctgtc ttacggcgtg
      601 cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
      661 cccgaaggct acgtccagga gcgcaccatc tccttcaagg acgacggcag ctacaggacc
      721 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcgtcgagct gaagggcatc
      781 gacttcaggg aggacggcaa catcctgggg cacaagctgg ggtacagcat gaacgtgtgg
      841 gacgcgtata tcacggccga
//
