LOCUS       sequence-57602-e         915 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Ding Y, Ai HW, Hoi H, Campbell RE
  TITLE     Forster resonance energy transfer-based biosensors for 
            multiparameter ratiometric imaging of Ca2+ dynamics and caspase-3 
            activity in single cells.
  JOURNAL   Anal Chem. 2011 Dec 15;83(24):9687-93. doi: 10.1021/ac202595g. Epub 
            2011 Nov 28.
  PUBMED    22080726
REFERENCE   2  (bases 1 to 915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/ac202595g";
            journalName: "Anal Chem"; date: "2011-12-15- 15"; volume: "83"; 
            issue: "24"; pages: "9687-93"
COMMENT     Sequence Label: pmAmetrine1.2
FEATURES             Location/Qualifiers
     source          1..915
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             80..97
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             101..133
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(137..145,146..160)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     primer_bind     complement(221..242)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gagctttttt cgcaactctc tactgtttct ccatacccgt tttttgggct aacaggagga
       61 attaaccatg gggggttctc atcatcatca tcatcatggt atggctagca tgactggtgg
      121 acagcaaatg ggtcgggatc tgtacgacga tgacgataag gatccgagct cgagcatggt
      181 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
      241 cgtaaacggc cacaggttca gcgtgcgcgg cgagggcgag ggcgatgcca ccaacggcaa
      301 gctgatcctg aagttcatct gcacctccgg cgagctgccc gtgccctggc ccaccctcgt
      361 gaccaccctg tcttacggcg tgcagtgctt cgcccgctac cccgaccaca tgaagcagca
      421 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
      481 ggacgacggc agctacagga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
      541 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
      601 ggagtacaac atgaacgcgt gggacgcgta tatcacggcc gacaagcaga agaacggcat
      661 caaagcgaac ttcaagatcg agcacaacgt caaggacggc ggcgtgcagc tcgccgacgc
      721 gtaccagcag aacaccccca tcggcggcgg ccccgtgctg ctgcctgaca accactacct
      781 gagcttccag agcaagctgt tcaaagaccc ctacgagcag cgcgatcaca tggtcctgct
      841 ggagtttgtt accgccgccg ggataactcc cggcatggac gactgacata gatcaactgt
      901 gttgcgagaa aattc
//
