LOCUS       sequence-57623-e         895 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Hwang WY, Fu Y, Reyon D, Maeder ML, Tsai SQ, Sander JD, Peterson RT,
            Yeh JR, Joung JK
  TITLE     Efficient genome editing in zebrafish using a CRISPR-Cas system.
  JOURNAL   Nat Biotechnol. 2013 Jan 29. doi: 10.1038/nbt.2501.
  PUBMED    23360964
REFERENCE   2  (bases 1 to 895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2013 Jan 29. doi: 10.1038/nbt.2501."
COMMENT     Sequence Label: MLM3639
FEATURES             Location/Qualifiers
     source          1..895
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     32..51
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        32..50
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      58..67
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgtcagat ccgctagaga tccgcggccg ctaatacgac tcactatagg gagagccgcc
       61 accatggata agaaatactc aataggctta gatatcggca caaatagcgt cggatgggcg
      121 gtgatcactg atgaatataa ggttccgtct aaaaagttca aggttctggg aaatacagac
      181 cgccacagta tcaaaaaaaa tcttataggg gctcttttat ttgacagtgg agagacagcg
      241 gaagcgactc gtctcaaacg gacagctcgt agaaggtata cacgtcggaa gaatcgtatt
      301 tgttatctac aggagatttt ttcaaatgag atggcgaaag tagatgatag tttctttcat
      361 cgacttgaag agtctttttt ggtggaagaa gacaagaagc atgaacgtca tcctattttt
      421 ggaaatatag tagatgaagt tgcttatcat gagaaatatc caactatcta tcatctgcga
      481 aaaaaattgg tagattctac tgataaagcg gatttgcgct taatctattt ggccttagcg
      541 catatgatta agtttcgtgg tcattttttg attgagggag atttaaatcc tgataatagt
      601 gatgtggaca aactatttat ccagttggta caaacctaca atcaattatt tgaagaaaac
      661 cctattaacg caagtggagt agatgctaaa gcgattcttt ctgcacgatt gagtaaatca
      721 agacgattag aaaatctcat tgctcagctc cccggtgaga agaaaaatgg cttatttggg
      781 aatctcattg ctttgtcatt gggtttgacc cctaatttta aatcaaattt tgatttggca
      841 gaagatgcta aattacagct ttcaaaagat acttacgatg atgatttaga taatt
//
