LOCUS       sequence-57624-e         869 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Hoi H, Matsuda T, Nagai T, Campbell RE
  TITLE     Highlightable Ca(2+) Indicators for Live Cell Imaging.
  JOURNAL   J Am Chem Soc. 2012 Dec 26.
  PUBMED    23256581
REFERENCE   2  (bases 1 to 869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Am Chem 
            Soc. 2012 Dec 26."
COMMENT     Sequence Label: pTorPE-GR-GECO1.1
FEATURES             Location/Qualifiers
     source          1..869
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             73..84
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             239..256
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             260..292
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(296..304,305..319)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             347..403
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
ORIGIN
        1 tttttatcgc aactctctac tgtttctcca tacccgtttt ttgggctaac aggaggaatt
       61 aaccatgggt ttaaagagga gaaaggtcat gaacaataac gatctctttc aggcatcacg
      121 tcggcgtttt ctggcacaac tcggcagctt aaccgacgcc gggacgctgg ggccgtcatt
      181 gttaacgccg cgacgtgcga ctgcggcgca agcggcgact gacgcttcta gaggttctca
      241 tcatcatcat catcatggta tggctagcat gactggtgga cagcaaatgg gtcgggatct
      301 gtacgacgat gatgacaagg accccaccac aacggacgac tcatcacgtc gtaagtggaa
      361 taagacaggt cacgcggtca gagctatagg tcggctgagc tcaccccctg ggtacagcac
      421 agagaagatc tacgtgcggg acggtgagct gaagggcgac gtgaagatga agctgctgct
      481 gaagggcggc ggccactatc gctgcgactt ccgcaccacc tacaaggtca agcagaaggc
      541 cgtaaagctg cccgactacc acttcgtgga ccaccgcatc gagatcctga gccacgacga
      601 ggactacaac aaggtgaagc tgtacgagca tgccgtggcc cgccactccg ccgacagcgt
      661 ggacgagctg tacaagggtg gcagcggcgg cattgtgagc aagggcgagg agaccattat
      721 gagcgtgatc aagcctgaca tgaagatcaa gctgcgcatg gagggcaacg tgaacggcca
      781 cgccttcgtg atcgagggcg agggcagcgg caagcccttc aaaggcatcc agacaattga
      841 tttggaggtg aaggagggcg ccccgctgc
//
