LOCUS       sequence-57628-e         717 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  gRNA targeted to zebrafish gene apoea.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Hwang WY, Fu Y, Reyon D, Maeder ML, Tsai SQ, Sander JD, Peterson RT,
            Yeh JR, Joung JK
  TITLE     Efficient genome editing in zebrafish using a CRISPR-Cas system.
  JOURNAL   Nat Biotechnol. 2013 Jan 29. doi: 10.1038/nbt.2501.
  PUBMED    23360964
REFERENCE   2  (bases 1 to 717)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2013 Jan 29. doi: 10.1038/nbt.2501."
COMMENT     Sequence Label: Zebrafish-gRNA-0001
FEATURES             Location/Qualifiers
     source          1..717
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(144..219)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(238..256)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(385..401)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(385..401)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      449..480
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        481..583
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(657..676)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 aggtgagcca gtgagttgat tgcagtccag ttacgctgga gtctgaggct cgtcctgaat
       61 gatatgcgac cgccggaggg ttgcgtttga gacgggcgac agatccagtc gcgctgcntc
      121 tcgtcgatcc aagcttttta aaagcaccga ctcggtgcca ctttttcaag ttgataacgg
      181 actagcctta ttttaacttg ctatttctag ctctaaaaca gcggcttctt ggctcatcct
      241 atagtgagtc gtattagcta gcggtgcgag cggatcgagc agtgtcgatc actactggac
      301 cgcgagctgt gctgcgaccc gtgatcttac ggcattatac gtatgatcgg tccacgatca
      361 gctagattat ctagtcagct tgatgtcata gctgtttcct gaggctcaat actgaccatt
      421 taaatcatac ctgacctcca tagcagaaag tcaaaagcct ccgaccggag gcttttgact
      481 tgatcggcac gtaagaggtt ccaactttca ccataatgaa ataagatcac taccgggcgt
      541 attttttgag ttatcgagat tttcaggagc taaggaagct aaaatgagcc atattcaacg
      601 ggaaacgtct tgcttgaagc cgcgattaaa ttccaacatg gatgctgatt tatatgggta
      661 taaatgggct cgcgataatg tcgggcaatc aggtgcgaca atctatcgat tgtatgg
//
