LOCUS       sequence-57643-e         790 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Hoi H, Matsuda T, Nagai T, Campbell RE
  TITLE     Highlightable Ca(2+) Indicators for Live Cell Imaging.
  JOURNAL   J Am Chem Soc. 2012 Dec 26.
  PUBMED    23256581
REFERENCE   2  (bases 1 to 790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Am Chem 
            Soc. 2012 Dec 26."
COMMENT     Sequence Label: pTorPE-GR-GECO1.2
FEATURES             Location/Qualifiers
     source          1..790
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             74..85
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             240..257
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             306..320
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     CDS             348..404
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
ORIGIN
        1 gcttttttcg caactctcta ctgtttctcc atacccgttt tttgggctaa caggaggaat
       61 taaccatggg tttaaagagg agaaaggtca tgaacaataa cgatctcttt caggcatcac
      121 gtcggcgttt tctggcacaa ctcggcagct taaccgacgc cgggacgctg gggccgtcat
      181 tgttaacgcc gcgacgtgcg actgcggcgc aagcggcgac tgacgcttct agaggttctc
      241 atcatcatca tcatcatggt aaggctagca tgaccggtgg acagcaaatg ggccgggatc
      301 tgtgcgacga tgatgataag gatctcacca caatggtcga ctcatcacgt cgtaagtgga
      361 ataagacagg tcacgcagtc agagctatag gtcggctgag ctcaccccct gggtacagca
      421 cagagaagat ctacgtgcgg gacggtgagc tgaagggcga cgtgaagatg aagctgctgc
      481 tgaagggcgg cggccactat cgctgcgact tccgcaccac ctacaaggtc aagcagaagg
      541 ccgtaaagct gcccgactac cacttcgtgg accaccgcat cgagatcctg agccacgacg
      601 aggactacaa caaggtgaag ctgtacgagc atgccgtggc ccgccactcc accgacagcg
      661 tggacgagct gtacaagggt ggcagcggtg gcatggtgag caagggcgag gagaccatta
      721 tgagcgtgat caagcctgac atgaagatca agctgcgcat ggagggcaac gtgaacggcc
      781 acgccttcgt
//
