LOCUS       sequence-57644-e         839 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Yamagata M, Sanes JR
  TITLE     Transgenic strategy for identifying synaptic connections in mice by 
            fluorescence complementation (GRASP).
  JOURNAL   Front Mol Neurosci. 2012;5:18. Epub 2012 Feb 16.
  PUBMED    22355283
REFERENCE   2  (bases 1 to 839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Mol 
            Neurosci."; date: "2012"; volume: "5"; pages: "18. Epub 2012 Feb 16"
COMMENT     Sequence Label: pCMV-N1-mouse-superfolderGFP (mGRASP)
FEATURES             Location/Qualifiers
     source          1..839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    25..105
                     /label=MCS
                     /note="multiple cloning site"
     CDS             755..802
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
ORIGIN
        1 cgactgtttg tgaccgtcag atccgctagc gctaccggac tcagatctcg agctcaagct
       61 tcgaattctg cagtcgacgg taccgcgggc ccgggatcca ccggtcgcca ccatgtccaa
      121 aggagaagag ctgttcactg gagtggtacc aatacttgtg gagttggacg gagatgtgaa
      181 cggacacaaa ttttcagtcc gcggggaggg ggaaggggat gctactattg gcaagctgac
      241 gctcaaattc atctgtacca ccggaaaact ccctgtaccc tggcccacac tggtgacaac
      301 tctgacttac ggcgtgcaat gttttagccg atacccagac cacatgaaga ggcacgactt
      361 tttcaaaagc gcaatgcctg aaggatacgt acaggaaagg accatttctt ttaaagacga
      421 cgggaagtac aaaacccggg cagtggtgaa gtttgagggc gataccctcg tcaataggat
      481 cgaattgaag ggaactgact tcaaagaaga tggcaacatc ctgggtcaca agcttgagta
      541 taactttaac tcccacaacg tgtatattac agccgacaaa cagaagaatg gaattaaggc
      601 taacttcact gtcagacaca atgtcgaaga tggctccgtg cagctcgccg atcactatca
      661 acagaatact cctatcgggg acggcccagt cctgctgccc gacaaccact acctgagtac
      721 ccagactgtt ctgagcaaag atccgaacga gaagcgcgac cacatggtgc tgcatgagta
      781 tgtcaacgct gcgggaatta ccctcggcat ggacgagctg tacaagtaaa gcggccgcg
//
