LOCUS       sequence-57648-e         655 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre-activated AAV expression of halorhodopsin 2.0 (eNpHR) fused to 
            EYFP for optogenetic inhibition.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 655)
  TITLE     Cre-dependent expression of halorhodopsin
REFERENCE   2  (bases 1 to 655)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAAV-double floxed-eNpHR-EYFP-WPRE-pA
FEATURES             Location/Qualifiers
     source          1..655
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     repeat_region   118..247
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        367..578
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
ORIGIN
        1 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
       61 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgtcct
      121 gcaggcagct gcgcgctcgc tcgctcactg aggccgcccg ggcgtcgggc gacctttggt
      181 cgcccggcct cagtgagcga gcgagcgcgc agagagggag tggccaactc catcactagg
      241 ggttcctgcg gccgcacgcg taagctttgc aaagatggat aaagttttaa acagagagga
      301 atctttgcag ctaatggacc ttctaggtct tgaaaggagt gggaattggc tccggtgccc
      361 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
      421 attgaaccgg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
      481 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
      541 acgttctttt tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc
      601 gcgggcctgg cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccac
//
