LOCUS       sequence-57670-e         907 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Su PC, Si W, Baker DL, Berger BW
  TITLE     High-Yield membraneprotein expression fromE. coliusing an engineered
            outer membrane protein F (OmpF) fusion.
  JOURNAL   Protein Sci. 2013 Jan 24. doi: 10.1002/pro.2224.
  PUBMED    23345122
REFERENCE   2  (bases 1 to 907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Sci. 2013 Jan 24. doi: 10.1002/pro.2224."
COMMENT     Sequence Label: pOmpF
FEATURES             Location/Qualifiers
     source          1..907
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             23..45
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             64..81
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             523..540
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             605..622
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(675..693)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      689..736
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tacaatttcc ctctagaata attttgttta actttaagaa ggagatatac catgggcagc
       61 agccatcatc atcatcatca cgcagaaatc tataacaaag atggcaacaa agtagatctg
      121 tacggtaaag ctgttggtct gcattatttt tccaagggta acggtgaaaa cagttacggt
      181 ggcaatggcg acatgaccta tgcccgtctt ggttttaaag gggaaactca aatcaattcc
      241 gatctgaccg gttatggtca gtgggaatat aacttccagg gtaacaactc tgaaggcgct
      301 gacgctcaaa ctggtaacaa aacgcgtctg gcattcgcgg gtcttaaata cgctgacgtt
      361 ggttctttcg attacggccg taactacggt gtggtttatg atgcactggg ttacaccgat
      421 atgctgccag aatttggtgg tgatactgca tacagcgatg acttcttcgt tggtcgtgtt
      481 ggcggggttg ctacctatcg taactccggc ggcggcggcg gcctggtgcc gcgcggcagc
      541 ggtaccacta gtgctagcgg atccgaattc gagctccgtc gacaagcttg cggccgcact
      601 cgagcaccac caccaccacc actgagatcc ggctgctaac aaagcccgaa aggaagctga
      661 gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt
      721 cttgaggggt tttttgctga aaggaggaac tatatccgga ttggcgaatg ggacgcgccc
      781 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
      841 gccagcgccc tagcgcccgc tcctttcgct tttcttccct tcctttctcg ccacggtcng
      901 ccggctt
//
