LOCUS       sequence-57674-e         810 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Rhee HW, Zou P, Udeshi ND, Martell JD, Mootha VK, Carr SA, Ting AY
  TITLE     Proteomic Mapping of Mitochondria in Living Cells via Spatially 
            Restricted Enzymatic Tagging.
  JOURNAL   Science. 2013 Jan 31.
  PUBMED    23371551
REFERENCE   2  (bases 1 to 810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2013 Jan 31."
COMMENT     Sequence Label: pcDNA3-APX(W41F)-NES
FEATURES             Location/Qualifiers
     source          1..810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..27
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             28..774
                     /codon_start=1
                     /product="soybean ascorbate peroxidase, engineered to serve
                     as a monomeric tag for electron microscopy and live-cell 
                     proteomics (Lam et al., 2015)"
                     /label=APEX2
                     /note="more active than the original APEX due to the A134P 
                     mutation"
                     /translation="GKSYPTVSADYQKAVEKAKKKLRGFIAEKRCAPLMLRLAFHSAGT
                     FDKGTKTGGPFGTIKHPAELAHSANNGLDIAVRLLEPLKAEFPILSYADFYQLAGVVAV
                     EVTGGPEVPFHPGREDKPEPPPEGRLPDATKGSDHLRDVFGKAMGLTDQDIVALSGGHT
                     IGAAHKERSGFEGPWTSNPLIFDNSYFTELLSGEKEGLLQLPSDKALLSDPVFRPLVDK
                     YAADEDAFFADYAEAHQKLSELGFADA"
     CDS             781..807
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
ORIGIN
        1 atggactaca aggatgacga cgataaggga aagtcttacc caactgtgag tgctgattac
       61 cagaaggccg ttgagaaggc gaagaagaag ctcagaggct tcatcgctga gaagagatgc
      121 gctcctctaa tgctccgttt ggcattccac tctgctggaa cctttgacaa gggcacgaag
      181 accggtggac ccttcggaac catcaagcac cctgccgaac tggctcacag cgctaacaac
      241 ggtcttgaca tcgctgttag gcttttggag ccactcaagg cggagttccc tattttgagc
      301 tacgccgatt tctaccagtt ggctggcgtt gttgccgttg aggtcacggg tggacctgaa
      361 gttccattcc accctggaag agaggacaag cctgagccac caccagaggg tcgcttgccc
      421 gatgccacta agggttctga ccatttgaga gatgtgtttg gcaaagctat ggggcttact
      481 gaccaagata tcgttgctct atctgggggt cacactattg gagctgcaca caaggagcgt
      541 tctggatttg agggtccctg gacctctaat cctcttattt tcgacaactc atacttcacg
      601 gagttgttga gtggtgagaa ggaaggtctc cttcagctac cttctgacaa ggctcttttg
      661 tctgaccctg tattccgccc tctcgttgat aaatatgcag cggacgaaga tgccttcttt
      721 gctgattacg ctgaggctca ccaaaagctt tccgagcttg ggtttgctga tgccctgcag
      781 ctgccccccc tggagcgcct gaccctggac
//
