LOCUS       sequence-57695-e         897 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Kopan R, Schroeter EH, Weintraub H, Nye JS
  TITLE     Signal transduction by activated mNotch: importance of proteolytic 
            processing and its regulation by the extracellular domain.
  JOURNAL   Proc Natl Acad Sci U S A. 1996 Feb 20;93(4):1683-8.
  PUBMED    8643690
REFERENCE   2  (bases 1 to 897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "1996-02-20"; volume: "93(4)"; pages: 
            "1683-8"
COMMENT     Sequence Label: pCS2 Notch1 Delta-EMV-6MT
FEATURES             Location/Qualifiers
     source          1..897
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     24..44
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        26..44
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     polyA_signal    157..291
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(194..213)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     248..267
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     315..331
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     315..331
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             complement(350..379)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(413..442)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(452..481)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(491..520)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(530..559)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(569..598)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(612..628)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(613..629)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
ORIGIN
        1 atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctgggta
       61 ccgggcccaa tgcattggcg ccgcggccgc gaattaaaaa acctcccaca cctccccctg
      121 aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat
      181 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
      241 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat ctacgtaata
      301 cgactcacta tagttctaga actagtggat cccccgggct gcaggaattc aagtcctctt
      361 cagaaatgag cttttgctcc atggtgaggt cgcccaagct ctccatttca ttcaagtcct
      421 cttcagaaat gagcttttgc tccatttcat tcaagtcctc ttcagaaatg agcttttgct
      481 ccatttcatt caagtcctct tcagaaatga gcttttgctc catttcattc aagtcctctt
      541 cagaaatgag cttttgctcc atttcattca agtcctcttc agaaatgagc ttttgctcca
      601 tagctttaaa tcgataccgt cgacctcgat gacgcgtcat cgagctgtcc aacaggcagc
      661 ccttgccatc ctgggacttc ttcctccgtg ccttgaggtc cttagcttcc ttgctaccac
      721 aagccagccc tttggtgctg ggcttgcggg ccttcttgcc ctgtgtggca gacttgagat
      781 tgcccaggta gccattgggc gagcagagtg tgggagacag agtgggtgtg ccacccaggg
      841 cagtgccatg cagctgtggg ctgcgcacca ggttgtactc atccaaaagc cgcacga
//
