LOCUS       sequence-57733-e         790 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Luciferase reporter for GI-ZFP2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Polstein LR, Gersbach CA
  TITLE     Light-inducible spatiotemporal control of gene activation by 
            customizable zinc finger transcription factors.
  JOURNAL   J Am Chem Soc. 2012 Oct 10;134(40):16480-3. doi: 10.1021/ja3065667. 
            Epub 2012 Sep 27.
  PUBMED    22963237
REFERENCE   2  (bases 1 to 790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/ja3065667";
            journalName: "J Am Chem Soc"; date: "2012-10-10- 10"; volume: "134";
            issue: "40"; pages: "16480-3"
COMMENT     Sequence Label: pGL3-Basic-9xSeq2-Luc
FEATURES             Location/Qualifiers
     source          1..790
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      28..37
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(67..91)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(88..126)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     misc_feature    333..424
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(365..384)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    438..486
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(754..775)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ggcctttctt tatgtttttg gcgtcttcca tggtggcttt accaacagta ccggaatgcc
       61 aagcttgatc tgacggttca ctaaacgagc tctgcttata tagacctccc accgtacacg
      121 cctaccagat ctcttttgca gtttctcgac cttttgcagt ttctcgacct tttgcagttt
      181 ctcgaccttt tgcagtttct cgaccttttg cagtttctcg accttttgca gtttctcgac
      241 cttttgcagt ttctcgacct tttgcagttt ctcgaccttt tgcagtttct cgagcccggg
      301 ctagcacgcg taagagctcg gtacctatcg atagagaaat gttctggcac ctgcacttgc
      361 actggggaca gcctattttg ctagtttgtt ttgtttcgtt ttgttttgat ggagagcgta
      421 tgttagtact atcgattcac acaaaaaacc aacacacaga tgtaatgaaa ataaagatat
      481 tttattgcgg ccgctccaag tacctcccgt accttaatat tacttactta tcatggtagc
      541 ttgggctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
      601 ctgaatggcg aatggcaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt
      661 tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca
      721 aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta
      781 aagaacgtgg
//
