LOCUS       sequence-57734-e         949 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Beta-catenin reporter - 3rd generation self-inactivating lentiviral 
            plasmid LEF-1/TCF-responsive promoter driven d2-eGFP (destabilized, 
            half-life of 2h)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 949)
  AUTHORS   Reya T, Duncan AW, Ailles L, Domen J, Scherer DC, Willert K, Hintz 
            L, Nusse R, Weissman IL
  TITLE     A role for Wnt signalling in self-renewal of haematopoietic stem 
            cells.
  JOURNAL   Nature. 2003 May 22. 423(6938):409-14.
  PUBMED    12717450
REFERENCE   2  (bases 1 to 949)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2003 May 22. 423(6938):409-14."
COMMENT     Sequence Label: Lentiviral-TOP-dGFP-reporter
FEATURES             Location/Qualifiers
     source          1..949
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      19..28
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      44..53
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    232..349
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(616..660)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gctcctcgcc cttgctcacc atggtggcga ccggtggatc atcccatggt ggctttacca
       61 acagtaccgg aatgccaagc ttacttagat cgcagatctc gagtaccgga tccattatat
      121 accctctaga cctttgatct tacccccttt gatcttaccc cctttgatcc tgcaggcatg
      181 caagcttcag ctgctcgagc ccgggctatc gaggctagtc tcgtgatcga taaaattttg
      241 aatttttgta atttgttttt gtaattcttt agtttgtatg tctgttgcta ttatgtctac
      301 tattctttcc cctgcactgt accccccaat cccccctttt cttttaaaag ttaaccgata
      361 ccgtcgagat ccgttcacta atcgaatgga tctgtctctg tctctctctc caccttcttc
      421 ttctattcct tcgggcctgt cgggtcccct cggggttggg aggtgggtct gaaacgataa
      481 tggtgaatat ccctgcctaa ctctattcac tatagaaagt acagcaaaaa ctattcttaa
      541 acctaccaag cctcctacta tcattatgaa taattttata taccacagcc aatttgttat
      601 gttaaaccaa ttccacaaac ttgcccattt atctaattcc aataattctt gttcattctt
      661 ttcttgctgg ttttgcgatt cttcaattaa ggagtgtatt aagcttgtgt aattgttaat
      721 ttctctgtcc cactccatcc aggtcgtgtg attccaaatc tgttccagag atttattact
      781 ccaactagca ttccaaggca cagcagtggt gcaaatgagt tttccagagc aaccccaaat
      841 ccccaggagc tgttgatcct ttaggtatct ttccacagcc aggattcttg cctggagctg
      901 cttgatgccc cagactgtga gttgcaacag atgctgttgc gcctcaata
//
