LOCUS       sequence-57735-e         879 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses GI-ZFP2 for light-inducible activation of gene expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Polstein LR, Gersbach CA
  TITLE     Light-inducible spatiotemporal control of gene activation by 
            customizable zinc finger transcription factors.
  JOURNAL   J Am Chem Soc. 2012 Oct 10;134(40):16480-3. doi: 10.1021/ja3065667. 
            Epub 2012 Sep 27.
  PUBMED    22963237
REFERENCE   2  (bases 1 to 879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/ja3065667";
            journalName: "J Am Chem Soc"; date: "2012-10-10- 10"; volume: "134";
            issue: "40"; pages: "16480-3"
COMMENT     Sequence Label: pcDNA3-GI-ZFP2
FEATURES             Location/Qualifiers
     source          1..879
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(373..393)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(400..423)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tagggccctc tagatcacgc gaagatgaat ctttgtatga gtggttcggt ggttccgctg
       61 ggcgaacttg cgcccgcata tatcacaagc gaatggtttc tcgccggtat gtgtgcggat
      121 gtgttcggac agcacgtcag atcttgaaaa gtttcgcata catatccggc actgaaaggg
      181 cttttcgcct gtgtggattt tagtgtggac ttgcaggtta atcttctgtg cgaatttccg
      241 accacagatg tcgcaggcaa acggcttttc cccggtatgc gtcctgatgt gaacgctcaa
      301 attatcgctc ctactaaaat ttctcatgca aattctacac tggaaaggcc tctctccggg
      361 ctcgaggcgg cccaccttcc tcttcttctt gggggccatc ttgtcatcgt catccttgta
      421 atcggcggcc gcttgggaca aggatatagt acagccgagt tcccgagccg cagtgtcgag
      481 aaactgagta ggcatagttg tggagagcaa gctgtgagct tcccagttta aacagttttg
      541 gatatcggct ttccagtcta ttgaggcggc gttgaagaat cgataggacg gactattcat
      601 tccgttcttc tctgttgttg gcagttttgg agttactttg atgggtatgg agctttggtt
      661 catgatatca cgtagtactg acgtgcttaa ggcacgtacg tgtgcacttg ggtgtgaaag
      721 gcaccgtatt gttgctggta gacgacactt caatagattg gataaaccgt cgaccactgc
      781 tagtccagat ggcccccaag ctagcaccgg ctgtattgct cttgccgtgg cttcaagtag
      841 ctcaagttga ggtaatgtac aagcttctcc atcaacaag
//
