LOCUS       sequence-57738-e         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Seeliger JC, Topp S, Sogi KM, Previti ML, Gallivan JP, Bertozzi CR
  TITLE     A riboswitch-based inducible gene expression system for 
            mycobacteria.
  JOURNAL   PLoS One. 2012;7(1):e29266. Epub 2012 Jan 18.
  PUBMED    22279533
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(1)"; pages: "e29266. Epub 2012 Jan 18"
COMMENT     Sequence Label: pST5832
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(17..190)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
                     DRPSQQLRSLNGE"
     primer_bind     139..161
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     153..170
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     154..170
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 accaggcaaa gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc
       61 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt
      121 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gaatccgtaa
      181 tcatggtcat cttgttgcct ccttagcagg gtgctgccaa gggcatcaag acgatgctgg
      241 tatcaccggt acctatagtg agtcgtatcc cggtcgcgag tgccaacgtt attcttagca
      301 ctcgcctatg ccgagtgcaa gaagccccgc accgggtcat gcccctcgtt cgaccttgtc
      361 ctcggccctc cgatccgggt gagtatgttc ggcccatgac cgccaacgac aacaagaccc
      421 gtaaatggtc ggccgcagac gtccccgatc aaagcgggcg cgttgtggtc acctctagat
      481 ttaaagatct ggtaccgcgg ccgcgccacg gatgccacca caggcactac aacggagttc
      541 gccacgtaca tcaccacaac caccgattct ggcggtgagc tccccgatat tcagcggaaa
      601 tggcttggta tcgaccaaga ttcgtagaac cccgtctcgt ctggctggta ttcaaaacgg
      661 gcgcaacgaa acacgcaacg agacaggcat ggcccaaacc agaaaactag cgtctaccag
      721 gacttttacc tgtccgaccc gttgcaacgg aaccccccac ggaacccccg cgacacccgc
      781 tccccaattg cgttagaaca gcggtggatt gtcggcttcg ttgtgggcct tttgagccgc
      841 ttcctgttct gccgcacgct ctttcctcgc ccgatagccg agtcgcta
//
