LOCUS       sequence-57750-e        9091 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9091)
  AUTHORS   Navaroli DM, Bellve KD, Standley C, Lifshitz LM, Cardia J, Lambright
            D, Leonard D, Fogarty KE, Corvera S
  TITLE     Rabenosyn-5 defines the fate of the transferrin receptor following 
            clathrin-mediated endocytosis.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Feb 21;109(8):E471-80. Epub 2012 Jan 
            30.
  PUBMED    22308388
REFERENCE   2  (bases 1 to 9091)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2012-02-21"; volume: "109(8)"; pages: 
            "E471-80. Epub 2012 Jan 30"
COMMENT     Sequence Label: TagRFP-T-EEA1
FEATURES             Location/Qualifiers
     source          1..9091
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      607..616
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             613..1344
                     /codon_start=1
                     /product="photostable variant of TagRFP (Shaner et al., 
                     2008)"
                     /label=TagRFP-T
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRTD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
     CDS             1354..5589
                     /codon_start=1
                     /product="human early endosome antigen 1"
                     /label=EEA1
                     /translation="MLRRILQRTPGRVGSQGSDLDSSATPINTVDVNNESSSEGFICPQ
                     CMKSLGSADELFKHYEAVHDAGNDSGHGGESNLALKRDDVTLLRQEVQDLQASLKEEKW
                     YSEELKKELEKYQGLQQQEAKPDGLVTDSSAELQSLEQQLEEAQTENFNIKQMKDLFEQ
                     KAAQLATEIADIKSKYDEERSLREAAEQKVTRLTEELNKEATVIQDLKTELLQRPGIED
                     VAVLKKELVQVQTLMDNMTLERERESEKLKDECKKLQSQYASSEATISQLRSELAKGPQ
                     EVAVYVQELQKLKSSVNELTQKNQTLTENLLKKEQDYTKLEEKHNEESVSKKNIQATLH
                     QKDLDCQQLQSRLSASETSLHRIHVELSEKGEATQKLKEELSEVETKYQHLKAEFKQLQ
                     QQREEKEQHGLQLQSEINQLHSKLLETERQLGEAHGRLKEQRQLSSEKLMDKEQQVADL
                     QLKLSRLEEQLKEKVTNSTELQHQLDKTKQQHQEQQALQQSTTAKLREAQNDLEQVLRQ
                     IGDKDQKIQNLEALLQKSKENISLLEKEREDLYAKIQAGEGETAVLNQLQEKNHTLQEQ
                     VTQLTEKLKNQSESHKQAQENLHDQVQEQKAHLRAAQDRVLSLETSVNELNSQLNESKE
                     KVSQLDIQIKAKTELLLSAEAAKTAQRADLQNHLDTAQNALQDKQQELNKITTQLDQVT
                     AKLQDKQEHCSQLESHLKEYKEKYLSLEQKTEELEGQIKKLEADSLEVKASKEQALQDL
                     QQQRQLNTDLELRATELSKQLEMEKEIVSSTRLDLQKKSEALESIKQKLTKQEEEKQIL
                     KQDFETLSQETKIQHEELNNRIQTTVTELQKVKMEKEALMTELSTVKDKLSKVSDSLKN
                     SKSEFEKENQKGKAAILDLEKTCKELKHQLQVQMENTLKEQKELKKSLEKEKEASHQLK
                     LELNSMQEQLIQAQNTLKQNEKEEQQLQGNINELKQSSEQKKKQIEALQGELKIAVLQK
                     TELENKLQQQLTQAAQELAAEKEKISVLQNNYEKSQETFKQLQSDFYGRESELLATRQD
                     LKSVEEKLSLAQEDLISNRNQIGNQNKLIQELKTAKATLEQDSAKKEQQLQERCKALQD
                     IQKEKSLKEKELVNEKSKLAEIEEIKCRQEKEITKLNEELKSHKLESIKEITNLKDAKQ
                     LLIQQKLELQGKADSLKAAVEQEKRNQQILKDQVKKEEEELKKEFIEKEAKLHSEIKEK
                     EVGMKKHEENEAKLTMQITALNENLGTVKKEWQSSQRRVSELEKQTDDLRGEIAVLEAT
                     VQNNQDERRALLERCLKGEGEIEKLQTKVLELQRKLDNTTAAVQELGRENQSLQIKHTQ
                     ALNRKWAEDNEVQNCMACGKGFSVTVRRHHCRQCGNIFCAECSAKNALTPSSKKPVRVC
                     DACFNDLQG"
     polyA_signal    5879..6000
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5916..5935)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5970..5989
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(6007..6462)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6144..6165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6356..6375
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6489..6593
                     /gene="bla"
                     /label=AmpR promoter
     promoter        6595..6952
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6595..6786
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6618..6638)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6803..6938
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6987..7781
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             6987..7781
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7041..7060)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7651..7670
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(7969..7988)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    8013..8060
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      8389..8977
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8878..8897
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgtc taagggcgaa gagctgatta aggagaacat gcacatgaag
      661 ctgtacatgg agggcaccgt gaacaaccac cacttcaagt gcacatccga gggcgaaggc
      721 aagccctacg agggcaccca gaccatgaga atcaaggtgg tcgagggcgg ccctctcccc
      781 ttcgccttcg acatcctggc taccagcttc atgtacggca gcagaacctt catcaaccac
      841 acccagggca tccccgattt ctttaagcag tccttccctg agggcttcac atgggagaga
      901 gtcaccacat acgaagacgg gggcgtgctg accgctaccc aggacaccag cctccaggac
      961 ggctgcctca tctacaacgt caagatcaga ggggtgaact tcccatccaa cggccctgtg
     1021 atgcagaaga aaacactcgg ctgggaggcc aacaccgaga tgctgtaccc cgctgacggc
     1081 ggcctggaag gcagaaccga catggccctg aagctcgtgg gcgggggcca cctgatctgc
     1141 aacttcaaga ccacatacag atccaagaaa cccgctaaga acctcaagat gcccggcgtc
     1201 tactatgtgg accacagact ggaaagaatc aaggaggccg acaaagagac ctacgtcgag
     1261 cagcacgagg tggctgtggc cagatactgc gacctcccta gcaaactggg gcacaaactt
     1321 aatggcatgg acgagctgta caagactcga gaaatgttaa ggaggatttt acagaggact
     1381 cctgggagag ttggctctca aggttctgat ttagattcat cagcaactcc tataaacaca
     1441 gtggacgtca ataatgaaag ctcttcagaa ggtttcatat gtccccagtg tatgaaatct
     1501 cttggatctg ctgatgaact tttcaaacat tatgaagctg ttcatgatgc tggtaatgac
     1561 tcaggtcatg gaggagagtc taatcttgct ttgaagcgag atgatgtaac actgctcaga
     1621 caagaggtcc aagacctaca ggcttcactt aaggaagaaa aatggtactc ggaagaatta
     1681 aagaaggaat tagaaaaata tcaagggctg cagcagcaag aggccaaacc tgatgggttg
     1741 gtgactgatt catcagcaga actacagtct ttggaacagc aattagaaga agcccaaaca
     1801 gaaaatttta atattaagca aatgaaagac ttatttgaac agaaagcagc ccaacttgct
     1861 actgaaattg cagatataaa gtcaaagtat gatgaagaaa ggagtcttcg agaagctgct
     1921 gaacaaaaag tgacacgtct gacagaagaa ttaaacaaag aggcaactgt aattcaagat
     1981 ctgaagacgg aactgcttca gagacctggt atagaagatg ttgccgtgct aaagaaagaa
     2041 ctggtccaag ttcaaacact aatggataac atgaccttgg aacgtgagcg agaatctgaa
     2101 aaactcaaag atgaatgcaa aaaattgcag tcacaatatg ctagctcaga ggccacaata
     2161 agccagctaa ggagtgaact tgccaaaggc ccccaggaag ttgctgtata tgtacaggaa
     2221 ctacaaaaac tgaaaagttc agttaatgaa ttaacacaaa aaaatcagac cttgacagaa
     2281 aacttgctga aaaaagaaca agactatact aagttagagg agaaacataa tgaagaatct
     2341 gtgagtaaaa agaatattca ggcaaccctt catcaaaaag acctagattg tcaacagctt
     2401 cagtcaagat tgtctgcatc tgaaacctca ctgcatagaa tacatgtaga actaagtgaa
     2461 aaaggagaag ctactcaaaa gctcaaagaa gaattatctg aggtagagac caagtaccag
     2521 catctaaagg cggagtttaa gcagctacaa caacagagag aagaaaagga gcagcatggg
     2581 ttacaactcc aaagtgaaat taatcaatta catagcaaac ttctagagac agagcgccaa
     2641 ctaggggaag ctcatggtag gctgaaggaa cagagacagc tttcaagtga aaagttgatg
     2701 gataaagaac aacaagtggc tgatttacaa ctcaaacttt ctcggttaga agagcagttg
     2761 aaggaaaaag ttacaaattc tacagaattg cagcatcaat tagataaaac aaagcaacag
     2821 catcaagaac aacaggctct tcagcaaagc accacggcaa aacttcgaga agctcagaat
     2881 gatttggaac aagttctacg tcaaattggc gataaggacc aaaagatcca gaaccttgaa
     2941 gctttattac agaagagtaa agaaaatatt tcattactag aaaaagaaag agaagatctt
     3001 tatgcaaaaa ttcaggctgg tgaaggagag actgctgttc ttaaccagtt acaagaaaaa
     3061 aaccatacac tacaggagca agtaactcaa ctaacagaga agctgaagaa tcagtcagaa
     3121 agtcataaac aagcccagga gaatttgcat gaccaggtac aagagcagaa ggcacatctt
     3181 agagctgcac aagaccgtgt cctttcccta gaaactagtg tcaatgaatt aaatagtcaa
     3241 ttaaatgaaa gcaaggagaa ggtctcccag cttgacatac agattaaagc caaaaccgaa
     3301 ctattactat cagcagaagc agcaaaaact gctcaaagag ctgatcttca gaatcatttg
     3361 gacacagctc aaaatgcatt acaagataaa cagcaggagt taaataagat tactactcag
     3421 ttggatcagg tcactgcaaa gttacaagac aagcaagaac attgcagtca gctggaaagt
     3481 catcttaaag aatataaaga gaaatacctc tctttagaac agaaaaccga agagctagaa
     3541 ggtcaaatta agaaactaga agctgatagt cttgaagtta aagcaagcaa ggagcaggct
     3601 ttgcaagatc tacaacagca aagacagctg aacacagatt tagagctcag agccacagaa
     3661 ttgagtaaac aacttgaaat ggagaaggaa atagtatcca gtacaagatt ggatctacag
     3721 aaaaaatctg aagcccttga aagtatcaag caaaagctta ccaagcaaga ggaagaaaaa
     3781 caaatcctga aacaagattt tgaaacttta agtcaagaaa caaagattca gcatgaggaa
     3841 ttgaataaca gaattcaaac aacagtaaca gaactacaaa aagtgaaaat ggagaaagaa
     3901 gctttaatga cagagctttc tacagtaaag gacaaactat caaaagtttc tgattctttg
     3961 aaaaactcta aaagtgaatt tgaaaaggag aatcagaaag gaaaagccgc tatattagac
     4021 ttggaaaaaa cttgcaaaga attaaagcat caacttcaag tgcagatgga aaacacactt
     4081 aaggaacaga aggaactgaa aaagtcactt gaaaaagaga aggaggcttc tcatcagttg
     4141 aaattggaac tcaattcaat gcaggaacaa cttatacagg cccagaatac tttaaaacaa
     4201 aatgaaaaag aagagcaaca acttcagggg aacataaatg agctaaagca atcaagtgaa
     4261 cagaagaaaa aacaaattga agcactccaa ggagagctta aaattgctgt tttacagaag
     4321 acagagcttg agaataaact acagcagcag ttaacacagg cagcccagga acttgcagca
     4381 gagaaagaga aaatatcagt attacaaaac aactatgaaa aaagtcagga aactttcaaa
     4441 cagcttcaat ctgatttcta tgggagggaa tctgaacttc tagccaccag gcaagatctt
     4501 aagtctgtag aagagaagct ttctctagca caggaggact tgatttcaaa cagaaatcaa
     4561 attggaaatc aaaataaatt gattcaagaa ctgaagactg ccaaggctac attggagcag
     4621 gattcagcaa agaaagaaca gcaattgcag gagcgatgta aagcactaca agacattcag
     4681 aaagaaaagt cactgaaaga aaaagaactg gtaaatgaga agtctaaatt ggcagagata
     4741 gaagaaatta aatgtagaca agaaaaagaa atcactaaac taaacgaaga actcaagtcc
     4801 cacaaactag aaagcataaa ggagataaca aatcttaaag atgctaaaca gcttctaatt
     4861 cagcagaaat tagaacttca aggaaaagcg gactccctga aggcagctgt tgaacaggag
     4921 aagagaaatc agcagatact aaaagaccag gtgaaaaagg aagaagagga gctgaagaaa
     4981 gaatttattg agaaagaagc taagttgcat tccgaaataa aagaaaagga agtaggaatg
     5041 aagaagcatg aagaaaatga ggctaaactt accatgcaga ttacagcatt aaatgaaaac
     5101 ttaggcactg tgaagaagga gtggcaatct agtcaacgga gagttagtga gcttgagaaa
     5161 caaacggatg acttacgggg tgaaattgca gtattagaag caacggttca gaataatcaa
     5221 gatgaaagga gagcactact ggaaagatgt cttaaaggag aaggtgaaat agaaaagctt
     5281 caaaccaaag tattagaatt gcaaagaaag ctggataata caactgcagc agtgcaggag
     5341 ctgggcagag aaaaccaatc acttcagatc aaacatacac aagcgttgaa tagaaagtgg
     5401 gccgaagaca atgaagtaca aaactgtatg gcctgtggga aaggcttttc agtaacagtg
     5461 agacggcatc actgccgaca gtgtggaaat atcttctgtg ctgaatgttc agccaaaaat
     5521 gccttaactc cttcctccaa gaagcctgtt cgtgtctgtg atgcatgttt caatgacttg
     5581 caaggataat gggttatcac aacttcagag taatattaca ctaacattag atttttaata
     5641 aatgtactta atagaggtct tggactacta tttggtttgg acactggttg caatactaga
     5701 ccaaatagga attagtatat tttggaatgt tatggatatc gcggccgctg gatccaccgg
     5761 atctagataa ctgatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
     5821 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
     5881 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     5941 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     6001 acgcgtaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
     6061 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
     6121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
     6181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
     6241 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
     6301 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
     6361 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
     6421 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc acttttcggg
     6481 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     6541 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtcctgagg
     6601 cggaaagaac cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     6661 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     6721 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     6781 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     6841 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     6901 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aagatcgatc
     6961 aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc
     7021 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct
     7081 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg
     7141 acctgtccgg tgccctgaat gaactgcaag acgaggcagc gcggctatcg tggctggcca
     7201 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc
     7261 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga
     7321 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc
     7381 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc
     7441 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg
     7501 ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct
     7561 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc
     7621 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc
     7681 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc
     7741 agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga
     7801 aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt
     7861 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
     7921 cggggatctc atgctggagt tcttcgccca ccctaggggg aggctaactg aaacacggaa
     7981 ggagacaata ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca
     8041 cggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc actctgtcga
     8101 taccccaccg agaccccatt ggggccaata cgcccgcgtt tcttcctttt ccccacccca
     8161 ccccccaagt tcgggtgaag gcccagggct cgcagccaac gtcggggcgg caggccctgc
     8221 catagcctca ggttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     8281 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     8341 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     8401 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     8461 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     8521 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     8581 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     8641 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     8701 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     8761 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     8821 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     8881 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     8941 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     9001 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     9061 ttctgtggat aaccgtatta ccgccatgca t
//
