LOCUS       sequence-57753-e         834 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Zawel L, Dai JL, Buckhaults P, Zhou S, Kinzler KW, Vogelstein B, 
            Kern SE
  TITLE     Human Smad3 and Smad4 are sequence-specific transcription 
            activators.
  JOURNAL   Mol Cell. 1998 Mar . 1(4):611-7.
  PUBMED    9660945
REFERENCE   2  (bases 1 to 834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            1998 Mar . 1(4):611-7."
COMMENT     Sequence Label: MBE6-Luc
FEATURES             Location/Qualifiers
     source          1..834
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      37..46
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(76..272)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      90..225
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(385..401)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(386..402)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     misc_feature    426..517
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(458..477)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    531..579
                     /note="synthetic polyadenylation signal"
ORIGIN
        1 atggcgccgg gcctttcttt atgtttttgg cgtcttccat ggtggcttta ccaacagtac
       61 cggaatgcca agctttttgc aaaagcctag gcctccaaaa aagcctcctc actacttctg
      121 gaatagctca gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcg
      181 atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg gagttagggg
      241 cgggactatg gttgctgact aattgagatg cagatcgcag atctcgagcc cgggctgcag
      301 gaattcgatc tagacatagt ctagacaggt gtctagacat agtctagaca ggtgtctaga
      361 catagtctag acaatcaagc ttatcgatac cgtcgacctc gagggggggc ccggtaccta
      421 tcgatagaga aatgttctgg cacctgcact tgcactgggg acagcctatt ttgctagttt
      481 gttttgtttc gttttgtttt gatggagagc gtatgttagt actatcgatt cacacaaaaa
      541 accaacacac agatgtaatg aaaataaaga tattttattg cggccgctcc aagtacctcc
      601 cgtaccttaa tattacttac ttatcatggt agcttgggct ggcgtaatag cgaagaggcc
      661 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggca aattgtaagc
      721 gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa
      781 taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat aggg
//
