LOCUS       sequence-57985-e         958 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Bar-Peled L, Schweitzer LD, Zoncu R, Sabatini DM
  TITLE     Ragulator Is a GEF for the Rag GTPases that Signal Amino Acid Levels
            to mTORC1.
  JOURNAL   Cell. 2012 Sep 14;150(6):1196-208. doi: 10.1016/j.cell.2012.07.032.
  PUBMED    22980980
REFERENCE   2  (bases 1 to 958)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2012.07"; journalName: "Cell"; date: "2012-09-14- 
            14"; volume: "150"; issue: "6"; pages: "1196-208"
COMMENT     Sequence Label: N1-p18-EGFP
FEATURES             Location/Qualifiers
     source          1..958
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      83..92
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      581..590
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(632..653)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(893..912)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcgctacc ggactcagat
       61 ctcgagctca agcttcgaat tcgccaccat ggggtgctgc tacagcagcg agaacgagga
      121 ctcggaccag gaccgagagg agcggaagct gctgctggac cctagcagcc cccctaccaa
      181 agctctcaat ggagccgagc ccaactacca cagcctgcct tccgctcgca ctgatgagca
      241 ggccctgctc tcttccatcc ttgccaagac agccagcaac atcattgatg tgtctgctgc
      301 agactcacag ggcatggagc agcatgagta catggaccgt gccaggcagt acagcacccg
      361 cttggctgtg ctgagcagca gcctgaccca ttggaagaag ctgccaccgc tgccgtctct
      421 taccagccag ccccaccaag tgctggccag tgagcccatc ccgttctctg atttgcagca
      481 ggtctccagg atagctgctt atgcctacag tgcactttct cagatccgtg tggacgcaaa
      541 agaggagctg gttgtacagt ttgggatccc accaccggtc gccaccatgg tgagcaaggg
      601 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
      661 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
      721 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct
      781 gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt
      841 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
      901 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatc
//
