LOCUS       sequence-57997-e         974 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 974)
  AUTHORS   Bar-Peled L, Schweitzer LD, Zoncu R, Sabatini DM
  TITLE     Ragulator Is a GEF for the Rag GTPases that Signal Amino Acid Levels
            to mTORC1.
  JOURNAL   Cell. 2012 Sep 14;150(6):1196-208. doi: 10.1016/j.cell.2012.07.032.
  PUBMED    22980980
REFERENCE   2  (bases 1 to 974)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2012.07"; journalName: "Cell"; date: "2012-09-14- 
            14"; volume: "150"; issue: "6"; pages: "1196-208"
COMMENT     Sequence Label: pRK5-HA-C7orf59
FEATURES             Location/Qualifiers
     source          1..974
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      27..36
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(400..426)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     regulatory      429..438
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(523..542)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        complement(555..573)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(556..573)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(783..807)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(804..903)
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(833..853)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ttataagctg caataaacaa gttgggccat ggcggccaag ctggggatcc ttacttactt
       61 agcggccgct cagacatcaa tgggctcccg acctcggttc tgcctcttca ccacaaacac
      121 cctctgtcct gacaccgtca ccagcagtgt gtgttctcca aagaccacag acaggcgctt
      181 gaagggcaca ttcatgccgc ggtgcagccg gaaaccgcag gctgtgctga ccagctcaga
      241 gatggcactg gctgcctgct catcattctc caggtcccca gatgacgcca gcactgcacc
      301 ttcactcagt accaggtagc cgagctggtc tgggattcgc tccagcccct gggtcagcgc
      361 agaagtcatc gtcgacccac caccaccacc attgagatct gcgtagtcag gcacatcata
      421 ggggtagccc atggtggcgc gaatggcctt ggtgaagaga ccgactgtcg gttcgggaat
      481 tcaatcgata gaaccgaggt gcagttggac ctgggagtgg acacctgtgg agagaaaggc
      541 aaagtggatg ttattctata gtgtcaccta aatcgtatgt gtatgataca taaggttatg
      601 tattaattgt agccgcgttc taacgaagcc aagggggtgg gcctatagac tctataggcg
      661 gtacttacgt cactcttggc acggggaatc cgcgttccaa tgcaccgttc ccggccgcgg
      721 aggctggatc ggtcccggtg tcttctatgg aggtcaaaac agcgtggatg gcgtctccag
      781 gcgatctgac ggttcactaa acgagctctg cttatataga cctcccaccg tacacgccta
      841 ccgcccattt gcgtcaatgg ggcggagttg ttacgacatt ttggaaagtc ccgttgattt
      901 tggtgccaaa acaaactccc attgacgtca atggggtgga gacttggaaa tccccgtgag
      961 tcaaaccgct atcc
//
