LOCUS       sequence-57999-e        1011 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Bar-Peled L, Schweitzer LD, Zoncu R, Sabatini DM
  TITLE     Ragulator Is a GEF for the Rag GTPases that Signal Amino Acid Levels
            to mTORC1.
  JOURNAL   Cell. 2012 Sep 14;150(6):1196-208. doi: 10.1016/j.cell.2012.07.032.
  PUBMED    22980980
REFERENCE   2  (bases 1 to 1011)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2012.07"; journalName: "Cell"; date: "2012-09-14- 
            14"; volume: "150"; issue: "6"; pages: "1196-208"
COMMENT     Sequence Label: pRK5-p18-FLAG
FEATURES             Location/Qualifiers
     source          1..1011
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      27..36
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(57..80)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      581..590
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(643..662)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        complement(675..693)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(676..693)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(903..927)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(924..975)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(953..973)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ttataagctg caataaacaa gttgggccat ggcggccaag ctggggatcc ttatcacttg
       61 tcatcgtcat ccttgtagtc acctccacct cctgcggccg ctgggatccc aaactgtaca
      121 accagctcct cttttgcgtc cacacggatc tgagaaagtg cactgtaggc ataagcagct
      181 atcctggaga cctgctgcaa atcagagaac gggatgggct cactggccag cacttggtgg
      241 ggctggctgg taagagacgg cagcggtggc agcttcttcc aatgggtcag gctgctgctc
      301 agcacagcca agcgggtgct gtactgcctg gcacggtcca tgtactcatg ctgctccatg
      361 ccctgtgagt ctgcagcaga cacatcaatg atgttgctgg ctgtcttggc aaggatggaa
      421 gagagcaggg cctgctcatc agtgcgagcg gaaggcaggc tgtggtagtt gggctcggct
      481 ccattgagag ctttggtagg ggggctgcta gggtccagca gcagcttccg ctcctctcgg
      541 tcctggtccg agtcctcgtt ctcgctgctg tagcagcacc ccatggtggc gtcgacgaat
      601 tcaatcgata gaaccgaggt gcagttggac ctgggagtgg acacctgtgg agagaaaggc
      661 aaagtggatg ttattctata gtgtcaccta aatcgtatgt gtatgataca taaggttatg
      721 tattaattgt agccgcgttc taacgaagcc aagggggtgg gcctatagac tctataggcg
      781 gtacttacgt cactcttggc acggggaatc cgcgttccaa tgcaccgttc ccggccgcgg
      841 aggctggatc ggtcccggtg tcttctatgg aggtcaaaac agcgtggatg gcgtctccag
      901 gcgatctgac ggttcactaa acgagctctg cttatataga cctcccaccg tacacgccta
      961 ccgcccattt gcgtcaatgg ggcggagttg ttacgacatt ttggaaagtc c
//
