LOCUS       sequence-58001-e         990 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Bar-Peled L, Schweitzer LD, Zoncu R, Sabatini DM
  TITLE     Ragulator Is a GEF for the Rag GTPases that Signal Amino Acid Levels
            to mTORC1.
  JOURNAL   Cell. 2012 Sep 14;150(6):1196-208. doi: 10.1016/j.cell.2012.07.032.
  PUBMED    22980980
REFERENCE   2  (bases 1 to 990)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2012.07"; journalName: "Cell"; date: "2012-09-14- 
            14"; volume: "150"; issue: "6"; pages: "1196-208"
COMMENT     Sequence Label: pRK5-HA-mp1
FEATURES             Location/Qualifiers
     source          1..990
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      27..36
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(475..501)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     regulatory      504..513
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(598..617)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        complement(630..648)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(631..648)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(858..882)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(879..978)
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(908..928)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ttataagctg caataaacaa gttgggccat ggcggccaag ctggggatcc ttacttactt
       61 agcggccgct taagaaactt ccacaacttg tctcagttct tcaaacaatg gagcaagttc
      121 cttttctagg ctgacaatta gtcctgtatt ggcactgctg ctggctatga aactcaccac
      181 caaaggtaaa cgattaaatt gaaccacctg gtaggtgtta tagtaacaga tgatactttt
      241 atttttggaa agtccaagtt tgcttccttg gtctgttgca agggcaaaag tggataagaa
      301 accaggtcgc aaagcatgct ctggagcatt gtcatttgcc actttaataa caggtactcc
      361 atctctatct gacacaacaa tggcatggag cccttcaaca cttggtaact ttttatacaa
      421 gaatcgcttt aggtcatccg ccatcgtcga cccaccacca ccaccattga gatctgcgta
      481 gtcaggcaca tcataggggt agcccatggt ggcgcgaatg gccttggtga agagaccgac
      541 tgtcggttcg ggaattcaat cgatagaacc gaggtgcagt tggacctggg agtggacacc
      601 tgtggagaga aaggcaaagt ggatgttatt ctatagtgtc acctaaatcg tatgtgtatg
      661 atacataagg ttatgtatta attgtagccg cgttctaacg aagccaaggg ggtgggccta
      721 tagactctat aggcggtact tacgtcactc ttggcacggg gaatccgcgt tccaatgcac
      781 cgttcccggc cgcggaggct ggatcggtcc cggtgtcttc tatggaggtc aaaacagcgt
      841 ggatggcgtc tccaggcgat ctgacggttc actaaacgag ctctgcttat atagacctcc
      901 caccgtacac gcctaccgcc catttgcgtc aatggggcgg agttgttacg acattttgga
      961 aagtcccgtt gattttggtg ccaaaacaac
//
