LOCUS       sequence-58002-e         983 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Bar-Peled L, Schweitzer LD, Zoncu R, Sabatini DM
  TITLE     Ragulator Is a GEF for the Rag GTPases that Signal Amino Acid Levels
            to mTORC1.
  JOURNAL   Cell. 2012 Sep 14;150(6):1196-208. doi: 10.1016/j.cell.2012.07.032.
  PUBMED    22980980
REFERENCE   2  (bases 1 to 983)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2012.07"; journalName: "Cell"; date: "2012-09-14- 
            14"; volume: "150"; issue: "6"; pages: "1196-208"
COMMENT     Sequence Label: pRK5-HA-HBXIP
FEATURES             Location/Qualifiers
     source          1..983
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      27..36
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(622..648)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     regulatory      651..660
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(745..764)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        complement(777..795)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(778..795)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 ttataagctg caataaacaa gttgggccat ggcggccaag ctggggatcc ttacttactt
       61 agcggccgct caagaggcca ttttgtgcac tgccaccgtg atgccatcgt gtttctggat
      121 cataatgttc ccattatctg attctagaca caccacagga atatcagtgg ggtcagaggt
      181 tagcttagct gcttgctggg ctagaacaga tatcactcca gcatgctcat ctgacagggt
      241 cccgcggcaa cccagattaa gtccttgtga atctgtgcac aggactccaa caatggaggg
      301 attcttcatt gtgtcttcca agtgctgctc caaggtcgcc tccatcccac ccaccgacca
      361 ctccggctca gaacccagcg gcacggcacg tccttctcca ccacaggcct cagtcacttg
      421 acgcgagcgg ggcgtggacc gtaacggcgc ctccaaaggg acaaaattga tcacggtgca
      481 acgtccgcgt tctttactgg aaaacatcac tgcgcttccg tcgcacagag cctgacgaag
      541 gcttgggctc cccgcgcggt gaccgtcgag gtgacctgca cctggctcca tcgtcgaccc
      601 accaccacca ccattgagat ctgcgtagtc aggcacatca taggggtagc ccatggtggc
      661 gcgaatggcc ttggtgaaga gaccgactgt cggttcggga attcaatcga tagaaccgag
      721 gtgcagttgg acctgggagt ggacacctgt ggagagaaag gcaaagtgga tgttattcta
      781 tagtgtcacc taaatcgtat gtgtatgata cataaggtta tgtattaatt gtagccgcgt
      841 tctaacgaag ccaagggggt gggcctatag actctatagg cggtacttac gtcactcttg
      901 gcacggggaa tccgcgttcc aatgcaccgt tcccggccgc ggaggctgga tcggtcccgg
      961 tgtcttctat ggaggtcaaa aca
//
