LOCUS       sequence-58005-e         990 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Bar-Peled L, Schweitzer LD, Zoncu R, Sabatini DM
  TITLE     Ragulator Is a GEF for the Rag GTPases that Signal Amino Acid Levels
            to mTORC1.
  JOURNAL   Cell. 2012 Sep 14;150(6):1196-208. doi: 10.1016/j.cell.2012.07.032.
  PUBMED    22980980
REFERENCE   2  (bases 1 to 990)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2012.07"; journalName: "Cell"; date: "2012-09-14- 
            14"; volume: "150"; issue: "6"; pages: "1196-208"
COMMENT     Sequence Label: pRK5-FLAG-HBXIP
FEATURES             Location/Qualifiers
     source          1..990
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      27..36
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(616..639)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(698..717)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        complement(730..748)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(731..748)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(958..982)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 ttataagctg caataaacaa gttgggccat ggcggccaag ctggggatcc ttacttactt
       61 agcggccgct caagaggcca ttttgtgcac tgccaccgtg atgccatcgt gtttctggat
      121 cataatgttc ccattatctg attctagaca caccacagga atatcagtgg ggtcagaggt
      181 tagcttagct gcttgctggg ctagaacaga tatcactcca gcatgctcat ctgacagggt
      241 cccgcggcaa cccagattaa gtccttgtga atctgtgcac aggactccaa caatggaggg
      301 attcttcatt gtgtcttcca agtgctgctc caaggtcgcc tccatcccac ccaccgacca
      361 ctccggctca gaacccagcg gcacggcacg tccttctcca ccacaggcct cagtcacttg
      421 acgcgagcgg ggcgtggacc gtaacggcgc ctccaaaggg acaaaattga tcacggtgca
      481 acgtccgcgt tctttactgg aaaacatcac tgcgcttccg tcgcacagag cctgacgaag
      541 gcttgggctc cccgcgcggt gaccgtcgag gtgacctgca cctggctcca tcgtcgacgc
      601 accaccacca ccacccttgt catcgtcatc cttgtagtcc atttccatgg tgaattcaat
      661 cgatagaacc gaggtgcagt tggacctggg agtggacacc tgtggagaga aaggcaaagt
      721 ggatgttatt ctatagtgtc acctaaatcg tatgtgtatg atacataagg ttatgtatta
      781 attgtagccg cgttctaacg aagccaaggg ggtgggccta tagactctat aggcggtact
      841 tacgtcactc ttggcacggg gaatccgcgt tccaatgcac cgttcccggc cgcggaggct
      901 ggatcggtcc cggtgtcttc tatggaggtc aaaacagcgt ggatggcgtc tccaggcgat
      961 ctgacggttc actaaacgag ctctgcttat
//
