LOCUS       sequence-58006-e         942 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 942)
  AUTHORS   Chisari M, Saini DK, Cho JH, Kalyanaraman V, Gautam N
  TITLE     G protein subunit dissociation and translocation regulate cellular 
            response to receptor stimulation.
  JOURNAL   PLoS One. 2009 Nov 11;4(11):e7797.
  PUBMED    19936219
REFERENCE   2  (bases 1 to 942)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-11-11"; volume: "4(11)"; pages: "e7797"
COMMENT     Sequence Label: YFP-Gamma 9-3
FEATURES             Location/Qualifiers
     source          1..942
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             22..39
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(320..337)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    326..550
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(683..702)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     893..914
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 atgactatac aaactagtat gcatcaccat caccatcacg cccaagagct cagcgagaag
       61 gaactcttaa agatggaggt agagcagctg aagaaggaag tgaagaaccc aagagctctg
      121 atttccaaaa caggaaagga aatcaaggat tatgtggagg ctgaagcagg aaacgaccct
      181 cttcttaaag gcatccctga gtccgagaac cccttccggg agaagaagtt cttctgtgcg
      241 ctcctctgag aattctgcag atatccagca cagtggcggc cgctcgagtc tagagggccc
      301 gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
      361 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
      421 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
      481 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
      541 ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg
      601 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
      661 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
      721 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
      781 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
      841 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
      901 ttgttccaaa ctggaacaac actcaaccct atctcggtct at
//
