LOCUS       sequence-58007-e         931 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Chisari M, Saini DK, Cho JH, Kalyanaraman V, Gautam N
  TITLE     G protein subunit dissociation and translocation regulate cellular 
            response to receptor stimulation.
  JOURNAL   PLoS One. 2009 Nov 11;4(11):e7797.
  PUBMED    19936219
REFERENCE   2  (bases 1 to 931)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-11-11"; volume: "4(11)"; pages: "e7797"
COMMENT     Sequence Label: mcherry-PH
FEATURES             Location/Qualifiers
     source          1..931
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             108..815
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(253..271)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     521..540
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg
       61 actcactata gggagaccca agctggctag cgtttaaact taagcttatg gtgagcaagg
      121 gcgaggagga taacatggcc atcatcaagg agttcatgcg cttcaaggtg cacatggagg
      181 gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg
      241 gcacccagac cgccaagctg aaggtgacca agggtggccc cctgcccttc gcctgggaca
      301 tcctgtcccc tcagttcatg tacggctcca aggcctacgt gaagcacccc gccgacatcc
      361 ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg
      421 aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc gagttcatct
      481 acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg cagaagaaga
      541 ccatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg
      601 agatcaagca gaggctgaag ctgaaggacg gcggccacta cgacgctgag gtcaagacca
      661 cctacaaggc caagaagccc gtgcagctgc ccggcgccta caacgtcaac atcaagttgg
      721 acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgaacgc gccgagggcc
      781 gccactccac cggcggcatg gacgagctgt acaaggaatt catggactcg ggccgggact
      841 tcctgaccct gcacggccta caggatgatg aggatctaca ggcgctgctg aagggcagcc
      901 agctcctgaa ggtgaagtcc agctcatgga g
//
