LOCUS       sequence-58008-e         969 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 969)
  AUTHORS   Bar-Peled L, Schweitzer LD, Zoncu R, Sabatini DM
  TITLE     Ragulator Is a GEF for the Rag GTPases that Signal Amino Acid Levels
            to mTORC1.
  JOURNAL   Cell. 2012 Sep 14;150(6):1196-208. doi: 10.1016/j.cell.2012.07.032.
  PUBMED    22980980
REFERENCE   2  (bases 1 to 969)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2012.07"; journalName: "Cell"; date: "2012-09-14- 
            14"; volume: "150"; issue: "6"; pages: "1196-208"
COMMENT     Sequence Label: pRK5-FLAG-RagB-D163N
FEATURES             Location/Qualifiers
     source          1..969
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             75..98
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ctttctctcc acaggtgtcc actcccaggt ccaactgcac ctcggttcta tcgattgaat
       61 tcaccatgga aatggactac aaggatgacg atgacaaggg tggtggtggt ggtgcgtcga
      121 cgatggaaga atctgactct gagaaaacga cggagaaaga aaatctgggg ccgagaatgg
      181 atccaccact aggggaaccg gaaggatcgc ttgggtgggt gctaccaaat acagccatga
      241 agaaaaaggt gctgttgatg ggtaaaagtg ggtctggtaa gaccagcatg aggtctatta
      301 tctttgcaaa ttatattgcc agagacacac gtcgccttgg cgcaacaatt gatgtagaac
      361 attctcatgt tcgatttctg ggaaacctgg tattgaacct gtgggattgt ggtgggcaag
      421 acaccttcat ggaaaattat ttcactagcc aacgggacaa catcttccga aatgtggagg
      481 ttctgattta tgtctttgat gtggagagcc gcgaactgga aaaggacatg cactattacc
      541 aatcatgcct ggaggccatt ctgcagaatt ctccagatgc caaaatattt tgcttggtac
      601 acaaaatgaa tctggtacag gaggatcaac gggacctgat ttttaaagag cgagaagaag
      661 atttgaggcg tttgtctcgc ccattggaat gttcttgttt ccgaacatct atctgggatg
      721 aaaccctcta taaggcttgg tccagcatag tttatcagct gattcccaat gttcagcagc
      781 tggaaatgaa cctaaggaat tttgctgaaa ttatcgaagc tgatgaagta cttctttttg
      841 agagagctac ttttctggta atttctcact atcagtgtaa agagcagcgt gatgcccata
      901 gatttgagaa aataagcaac attattaagc agttcaagct gagctgcagc aagctggctg
      961 cctctttcc
//
