LOCUS       sequence-58010-e         989 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 989)
  AUTHORS   Bar-Peled L, Schweitzer LD, Zoncu R, Sabatini DM
  TITLE     Ragulator Is a GEF for the Rag GTPases that Signal Amino Acid Levels
            to mTORC1.
  JOURNAL   Cell. 2012 Sep 14;150(6):1196-208. doi: 10.1016/j.cell.2012.07.032.
  PUBMED    22980980
REFERENCE   2  (bases 1 to 989)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2012.07"; journalName: "Cell"; date: "2012-09-14- 
            14"; volume: "150"; issue: "6"; pages: "1196-208"
COMMENT     Sequence Label: pRK5-FLAG-RagC-D181N
FEATURES             Location/Qualifiers
     source          1..989
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             75..98
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ctttctctcc acaggtgtcc actcccaggt ccaactgcac ctcggttcta tcgattgaat
       61 tcaccatgga aatggactac aaggatgacg atgacaaggg tggtggtggt ggtgcgtcga
      121 cgatgtccct gcagtacggg gcggaggaga cgcccctcgc cggcagttac ggcgcggccg
      181 attcgtttcc aaaggacttc ggctacggcg tggaggagga ggaagaggag gcggcggcgg
      241 cgggcggagg ggttggggca ggggcaggcg gtggctgtgg tccggggggc gctgacagct
      301 ccaagccgag gattctgctc atgggactcc ggcgcagcgg caagtcctcc atccagaagg
      361 tggtgtttca taagatgtca cccaacgaga ccctcttttt ggaaagtacc aacaagattt
      421 ataaggatga catttccaat agctcctttg tgaatttcca gatatgggat tttcctgggc
      481 aaatggactt ttttgaccca acctttgact atgagatgat cttcagggga acaggagcat
      541 tgatatacgt cattgacgca caggatgact acatggaggc tttaacaaga cttcacatta
      601 ctgtttctaa agcctacaaa gttaacccag acatgaattt tgaggttttt attcacaaag
      661 ttaatggtct gtctgatgat cacaaaatag aaacacagag ggacattcat caaagggcca
      721 atgatgacct tgcagatgct gggctagaaa aactccatct tagcttttat ctgactagta
      781 tctatgacca ttcaatattt gaagccttta gtaaggtggt gcagaaactc attccacaac
      841 tgccgacctt ggaaaaccta ttaaatatct ttatatcaaa ttcaggtatt gaaaaagctt
      901 ttctctttga tgttgtcagc aaaatctaca ttgcaacaga cagttcccct gtggatatgc
      961 aatcttatga actttgctgt gacatgatc
//
